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- Development of selection marker-free transgenic potato plants with enhanced tolerance to oxidative stress
- R Ahmad; Yun Hee Kim; Myoung Duck Kim; M N Phung; W I Chung; Haeng Soon Lee; Sang Soo Kwak; Suk Yoon Kwon
- Bibliographic Citation
- Journal of Plant Biology, vol. 51, no. 6, pp. 401-407
- Publication Year
- A binary vector devoid of a plant selection-marker gene (designated as pSSA-F) was constructed to overcome bio-safety concerns about genetically modified plants. This vector carried chloroplast-targeted superoxide dismutase (SOD) and ascorbate peroxidase (APX) genes under the control of an oxidative stress-inducible(SWPA2) promoter, and was utilized to transform potato (Solanum tuberosum L.). Integration of these foreign genes into transgenic plants was primarily performed via PCR with genomic DNA. Twelve marker-free transgenic lines were obtained by inoculating stem explants. The maximum transformation efficiency was 6.25% and averaged 2.2%. Successful integration of the SOD and APX genes rendered transgenic plants tolerant to methyl viologen-mediated oxidative stress at the leaf-disc and whole-plant levels. Our findings suggest that this technique for developing selection marker-free transgenic plants is feasible and can be employed with other crop species.
- genetic transformation; marker-free transgenic plants; oxidative stress; potato
- Appears in Collections:
- Division of Biomaterials Research > Plant Systems Engineering Research > 1. Journal Articles
Division of Biomaterials Research > 1. Journal Articles
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