DC Field | Value | Language |
---|---|---|
dc.contributor.author | H K Yoon | - |
dc.contributor.author | K C Sohn | - |
dc.contributor.author | J S Lee | - |
dc.contributor.author | Y J Kim | - |
dc.contributor.author | Jong Hwa Park | - |
dc.contributor.author | J M Yang | - |
dc.contributor.author | K H You | - |
dc.contributor.author | C D Kim | - |
dc.contributor.author | J H Lee | - |
dc.date.accessioned | 2017-04-19T09:13:09Z | - |
dc.date.available | 2017-04-19T09:13:09Z | - |
dc.date.issued | 2008 | - |
dc.identifier.issn | 0006-291X | - |
dc.identifier.uri | 10.1016/j.bbrc.2008.10.051 | ko |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/8808 | - |
dc.description.abstract | Terminal differentiation of skin keratinocytes is a vertically directed multi-step process that is tightly controlled by the sequential expression of a variety of genes. We previously investigated the gene expression profile and found that many of differentiation-related genes expressed in a temporally regulated manner. In this study, we attempted to find the hub-molecules and their intracellular signaling networks during keratinocyte differentiation using in silico analysis of data obtained from previous studies. We used protein-protein interaction prediction software called PSIMAP, and drew a hypothetical signaling network. We chose one candidate hub-molecule SHC1 that were predicted to link EGFR and MAPK signal, and then evaluated the protein-protein interactions experimentally. As predicted, SHC1 bound to the MEK1 in an EGF-regulated manner. Furthermore, SHC1 bound to the MEK1 and p38 MAPK in a keratinocyte differentiation dependent manner. These results demonstrate that in silico protein-protein interaction prediction system can be used to efficiently and cost-effectively select the experimental candidates. | - |
dc.publisher | Elsevier | - |
dc.title | Prediction and evaluation of protein-protein interaction in keratinocyte differentiation | - |
dc.title.alternative | Prediction and evaluation of protein-protein interaction in keratinocyte differentiation | - |
dc.type | Article | - |
dc.citation.title | Biochemical and Biophysical Research Communications | - |
dc.citation.number | 2 | - |
dc.citation.endPage | 667 | - |
dc.citation.startPage | 662 | - |
dc.citation.volume | 377 | - |
dc.contributor.affiliatedAuthor | Jong Hwa Park | - |
dc.contributor.alternativeName | 윤현경 | - |
dc.contributor.alternativeName | 손경철 | - |
dc.contributor.alternativeName | 이정숙 | - |
dc.contributor.alternativeName | 김유진 | - |
dc.contributor.alternativeName | 박종화 | - |
dc.contributor.alternativeName | 양준모 | - |
dc.contributor.alternativeName | 유관희 | - |
dc.contributor.alternativeName | 김창덕 | - |
dc.contributor.alternativeName | 이정훈 | - |
dc.identifier.bibliographicCitation | Biochemical and Biophysical Research Communications, vol. 377, no. 2, pp. 662-667 | - |
dc.identifier.doi | 10.1016/j.bbrc.2008.10.051 | - |
dc.subject.keyword | Keratinocyte | - |
dc.subject.keyword | MAPK | - |
dc.subject.keyword | Protein-protein interaction network | - |
dc.subject.keyword | PSIMAP | - |
dc.subject.keyword | SHC1 | - |
dc.subject.local | keratinocyte | - |
dc.subject.local | Keratinocytes | - |
dc.subject.local | Keratinocyte | - |
dc.subject.local | keratinocytes | - |
dc.subject.local | MAPK | - |
dc.subject.local | MAPKs | - |
dc.subject.local | Protein-protein interaction network | - |
dc.subject.local | PSIMAP | - |
dc.subject.local | SHC1 | - |
dc.description.journalClass | Y | - |
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