Prediction and evaluation of protein-protein interaction in keratinocyte differentiation

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dc.contributor.authorH K Yoon-
dc.contributor.authorK C Sohn-
dc.contributor.authorJ S Lee-
dc.contributor.authorY J Kim-
dc.contributor.authorJong Hwa Park-
dc.contributor.authorJ M Yang-
dc.contributor.authorK H You-
dc.contributor.authorC D Kim-
dc.contributor.authorJ H Lee-
dc.date.accessioned2017-04-19T09:13:09Z-
dc.date.available2017-04-19T09:13:09Z-
dc.date.issued2008-
dc.identifier.issn0006-291X-
dc.identifier.uri10.1016/j.bbrc.2008.10.051ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/8808-
dc.description.abstractTerminal differentiation of skin keratinocytes is a vertically directed multi-step process that is tightly controlled by the sequential expression of a variety of genes. We previously investigated the gene expression profile and found that many of differentiation-related genes expressed in a temporally regulated manner. In this study, we attempted to find the hub-molecules and their intracellular signaling networks during keratinocyte differentiation using in silico analysis of data obtained from previous studies. We used protein-protein interaction prediction software called PSIMAP, and drew a hypothetical signaling network. We chose one candidate hub-molecule SHC1 that were predicted to link EGFR and MAPK signal, and then evaluated the protein-protein interactions experimentally. As predicted, SHC1 bound to the MEK1 in an EGF-regulated manner. Furthermore, SHC1 bound to the MEK1 and p38 MAPK in a keratinocyte differentiation dependent manner. These results demonstrate that in silico protein-protein interaction prediction system can be used to efficiently and cost-effectively select the experimental candidates.-
dc.publisherElsevier-
dc.titlePrediction and evaluation of protein-protein interaction in keratinocyte differentiation-
dc.title.alternativePrediction and evaluation of protein-protein interaction in keratinocyte differentiation-
dc.typeArticle-
dc.citation.titleBiochemical and Biophysical Research Communications-
dc.citation.number2-
dc.citation.endPage667-
dc.citation.startPage662-
dc.citation.volume377-
dc.contributor.affiliatedAuthorJong Hwa Park-
dc.contributor.alternativeName윤현경-
dc.contributor.alternativeName손경철-
dc.contributor.alternativeName이정숙-
dc.contributor.alternativeName김유진-
dc.contributor.alternativeName박종화-
dc.contributor.alternativeName양준모-
dc.contributor.alternativeName유관희-
dc.contributor.alternativeName김창덕-
dc.contributor.alternativeName이정훈-
dc.identifier.bibliographicCitationBiochemical and Biophysical Research Communications, vol. 377, no. 2, pp. 662-667-
dc.identifier.doi10.1016/j.bbrc.2008.10.051-
dc.subject.keywordKeratinocyte-
dc.subject.keywordMAPK-
dc.subject.keywordProtein-protein interaction network-
dc.subject.keywordPSIMAP-
dc.subject.keywordSHC1-
dc.subject.localkeratinocyte-
dc.subject.localKeratinocytes-
dc.subject.localKeratinocyte-
dc.subject.localkeratinocytes-
dc.subject.localMAPK-
dc.subject.localMAPKs-
dc.subject.localProtein-protein interaction network-
dc.subject.localPSIMAP-
dc.subject.localSHC1-
dc.description.journalClassY-
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