Diversity of bacterial community in freshwater of Woopo wetland

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Title
Diversity of bacterial community in freshwater of Woopo wetland
Author(s)
K S Baik; S C Park; E M Kim; Kyung Sook Bae; J H Ahn; J O Ka; J Chun; C N Seong
Bibliographic Citation
Journal of Microbiology, vol. 46, no. 6, pp. 647-655
Publication Year
2008
Abstract
Diversity of bacterial community in water layer of Woopo wetland was investigated. Cultivable bacterial strains were isolated by the standard dilution plating technique and culture-independent 16S rRNA gene clones were obtained directly from DNA extracts of a water sample. Amplified rDNA restriction analysis (ARDRA) was applied onto both of the isolates and 16S rRNA gene clones. Rarefaction curves, coverage rate and diversity indices of ARDRA patterns were calculated. Representative isolates and clones of all the single isolate/clone phylotype were partially sequenced and analyzed phylogenetically. Sixty-four and 125 phylotypes were obtained from 203 bacterial isolates and 235 culture-independent 16S rRNA gene clones, respectively. Bacterial isolates were composed of 4 phyla, of which Firmicutes (49.8%) and Actinobacteria (32.0%) were predominant. Isolates were affiliated with 58 species. Culture-independent 16S rRNA gene clones were composed of 8 phyla, of which Proteobacteria (62.2%), Actinobacteria (15.5%), and Bacteroidetes (13.7%) were predominant. Diversity of 16S rRNA gene clones originated from cultivation-independent DNA extracts was higher than that of isolated bacteria. ⓒ 2008 The Microbiological Society of Korea and Springer-Verlag Berlin Heidelber GmbH.
Keyword
16S rRNA gene clonesBacterial isolatesDiversityPhylotypesWoopo wetland
ISSN
1225-8873
Publisher
Microbiological Society Korea
DOI
http://dx.doi.org/10.1007/s12275-008-0135-x
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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