Multiple-layer substrate zymography for detection of several enzymes in a single sodium dodecyl sulfate gel

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Title
Multiple-layer substrate zymography for detection of several enzymes in a single sodium dodecyl sulfate gel
Author(s)
Nack-Shick Choi; Bo-Hye Kim; C S Park; Yunjon Han; Hye-Won Lee; J H ChoiSeung Goo LeeJae Jun Song
Bibliographic Citation
Analytical Biochemistry, vol. 386, no. 1, pp. 121-122
Publication Year
2009
Abstract
We have developed a system to detect three hydrolytic enzymes (cellulase, lipase, and protease) using a single sodium dodecyl sulfate (SDS) gel and an electrotransfer system. After electrophoresis, proteins in the gel were transferred to three sandwiched substrate gels containing glycerol tributyrate, azo-carboxymethyl cellulose (Azo-CMC), and fibrin for detection of cellulase, lipase, and protease, respectively. We show that three cellulases (from a Paenibacillus sp. and two Bacillus sp. strains), one lipase (from a Staphylococcus sp.), and two proteases (from two Bacillus sp. strains) can be detected simultaneously with our zymogram system.
ISSN
0003-2697
Publisher
Elsevier
DOI
http://dx.doi.org/10.1016/j.ab.2008.11.007
Type
Article
Appears in Collections:
Jeonbuk Branch Institute > Microbial Biotechnology Research Center > 1. Journal Articles
Division of Biomaterials Research > 1. Journal Articles
Division of Bio Technology Innovation > SME Support Center > 1. Journal Articles
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