The effects of acrylamide on brain creatine kinase: Inhibition kinetics and computational docking simulation

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Title
The effects of acrylamide on brain creatine kinase: Inhibition kinetics and computational docking simulation
Author(s)
Z R Lu; H C Zou; Seongjin Park; Dae Ui Park; L Shi; Sang Ho Oh; Y D Park; Jong Hwa Park; F Zou
Bibliographic Citation
International Journal of Biological Macromolecules, vol. 44, no. 2, pp. 128-132
Publication Year
2009
Abstract
The occurrence of acrylamide is frequently observed in processed foods. Therefore, the harmful effects of acrylamide on metabolic enzymes are important to understand. We studied the inhibitory effects of acrylamide on the brain creatine kinase (CK-BB). We found that CK-BB was kinetically inactivated by acrylamide accompanied by the disruption of the hydrophobic surface. Acrylamide mainly interacted with the thiol (-SH) residue of CK-BB and resulted in alkylation. A computational docking simulation supported that acrylamide directly bound to the active site of CK-BB where cysteine and glycine residues interacted mainly. The inhibition kinetics combined with computational prediction can be useful in order to have insights into the mechanisms regarding environmentally hazardous factors at the molecular level.
Keyword
acrylamidebrain creatine kinaseinhibition kinetics
ISSN
0141-8130
Publisher
Elsevier
DOI
http://dx.doi.org/10.1016/j.ijbiomac.2008.11.003
Type
Article
Appears in Collections:
Korea Bioinformation Center > 1. Journal Articles
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