cDNA cloning and analysis of tissue-specific gene expression of rat urocortin II = 랫트 유로코틴2의 조직특이적 발현의 cDNA클론닝 및 분석

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dc.contributor.authorJung Il Chea-
dc.contributor.authorSeong Kyu Joo-
dc.contributor.authorMyung Kyu Lee-
dc.contributor.authorJ H Park-
dc.contributor.authorJ H Yoon-
dc.contributor.authorJ H Shim-
dc.contributor.authorD S Lee-
dc.date.accessioned2017-04-19T09:13:26Z-
dc.date.available2017-04-19T09:13:26Z-
dc.date.issued2009-
dc.identifier.issn0026-8933-
dc.identifier.uri10.1134/S0026893309010117ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/8879-
dc.description.abstractThe corticotropin-releasing hormone (CRH) family of neuropeptides includes CRH (a 41-amino acid hypothalamic peptide) and urocortin. Corticotropin- releasing factor (CRF), a peptide first isolated from mammalian, plays an important role in the regulation of the pituitary-adrenal axis, and in endocrine, autonomic, immune and behavioral responses to stress. In this study, we cloned rat urocortin II (UCN II) cDNA from rat mid-brain by RT-PCR. The rat UCN II clone contained an open reading frame (ORF) 109 amino acids which shared 90% and 63% homology with mouse and human homologues, respectively. The expression of UCN II mRNA is mainly distributed in bone marrow, ovary, uterus, hypophysis, adrenal gland, and skin. In this study, rat recombinant UCN was expressed in E. coli and purified in active form. Furthermore, purified recombinant UCN II protein specifically binds to CRF receptor 2 in rat ROS 17 / 2.8 and GH3 cells by flow-cytometry analysis. UCN II cDNA clone obtained in this study will be useful for further investigation on behavioral responses to stress in rats.-
dc.publisherSpringer-
dc.titlecDNA cloning and analysis of tissue-specific gene expression of rat urocortin II = 랫트 유로코틴2의 조직특이적 발현의 cDNA클론닝 및 분석-
dc.title.alternativecDNA cloning and analysis of tissue-specific gene expression of rat urocortin II-
dc.typeArticle-
dc.citation.titleMolecular Biology-
dc.citation.number1-
dc.citation.endPage87-
dc.citation.startPage82-
dc.citation.volume43-
dc.contributor.affiliatedAuthorJung Il Chea-
dc.contributor.affiliatedAuthorSeong Kyu Joo-
dc.contributor.affiliatedAuthorMyung Kyu Lee-
dc.contributor.alternativeName채정일-
dc.contributor.alternativeName주성규-
dc.contributor.alternativeName이명규-
dc.contributor.alternativeName박정현-
dc.contributor.alternativeName윤정헌-
dc.contributor.alternativeName-
dc.contributor.alternativeName이동석-
dc.identifier.bibliographicCitationMolecular Biology, vol. 43, no. 1, pp. 82-87-
dc.identifier.doi10.1134/S0026893309010117-
dc.subject.keywordurocortin II-
dc.subject.keywordrecombinant proteins-
dc.subject.keywordFACS-
dc.subject.localurocortin II-
dc.subject.localRecombinant Protein-
dc.subject.localrecombinant protein-
dc.subject.localrecombinant proteins-
dc.subject.localRecombinant protein-
dc.subject.localecombinant proteins-
dc.subject.localRecombinant proteins-
dc.subject.localFACS-
dc.description.journalClassY-
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