Enhancement of cadmium resistance by overexpression of BrMT3 in Arabidopsis = BrMT3 고발현에 의한 애기장대의 카드뮴 저항성 증진

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Title
Enhancement of cadmium resistance by overexpression of BrMT3 in Arabidopsis = BrMT3 고발현에 의한 애기장대의 카드뮴 저항성 증진
Author(s)
Sun Ha Kim; W Y Song; Young Ock Ahn; Haeng Soon Lee; Sang Soo Kwak; K S Choi
Bibliographic Citation
Journal of Plant Biotechnology, vol. 36, no. 1, pp. 68-74
Publication Year
2009
Abstract
We have previously demonstrated that overexpression and characterization of Brassica rapa type-l metallothionein gene (BrMT1) in Arabidopsis which showed enhanced resistance to cadmium and ROS. Here, we present the consistent study of our previous report about BrMTs. BrMT3 expressing DTY167 cells showed resistance to Zn and Pb as well as Cd. Thus, we have developed the BrMT3 overexpression Arabidopsis to enhance capacity for metal stresses. Successful expression and localization were achieved using the rubisco transit peptides of RbcS-BrMT3-GFP protein, which was confirmed by western blot analysis with the GFP antibody and green fluorescence signal from the chloroplast. BrMT3 overexpression Arabidopsis plants exhibited a higher resistance to cadmium compared to control plants. This result indicates that BrMT3 would be applicable to the development of plants with enhanced resistance against heavy metal stresses.
ISSN
1229-2818
Publisher
Korea Soc-Assoc-Inst
DOI
http://dx.doi.org/10.5010/JPB.2009.36.1.068
Type
Article
Appears in Collections:
Division of Research on National Challenges > Plant Systems Engineering Research > 1. Journal Articles
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