Selection of transgenic sweetpotato plants expressing 2-Cys peroxiredoxin with enhanced tolerance to oxidative stress = Peroxiredoxin 유전자 발현 산화스트레스 내성 형질전환 고구마의 선발

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dc.contributor.authorMyoung Duck Kim-
dc.contributor.authorKyoung-Sil Yang-
dc.contributor.authorSuk Yoon Kwon-
dc.contributor.authorS Y Lee-
dc.contributor.authorSang Soo Kwak-
dc.contributor.authorHaeng Soon Lee-
dc.date.accessioned2017-04-19T09:13:39Z-
dc.date.available2017-04-19T09:13:39Z-
dc.date.issued2009-
dc.identifier.issn1229-2818-
dc.identifier.uri10.5010/JPB.2009.36.1.075ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/8922-
dc.description.abstractIn order to develop transgenic sweetpotato plants [Ipomoea batatas (L.) Lam. cv. Yulmi] with enhanced tolerance to oxidative stress, we constructed transformation vectors expressing 2-Cys peroxiredoxin (Prx) gene under the control of the stress-inducible SWPA2 or enhanced 35S promoter (named as SP or EP). Transgenic sweetpotato plants were attempted to generate from embryogenic calli using an Agrobacterium-mediated transformation system. Embryogenic calli gave rise to somatic embryos and then converted into plantlets on MS medium containing 100 mg/L kanamycin. Transgenic plants were regenerated in the same medium. Southern blot analysis confirmed that the Prx gene was inserted into the genome of the plants. To further study we selected the transgenic plant lines with enhanced tolerance against methyl viologen (MV). When sweetpotato leaf discs were subjected to methyl MV at 20 μM, transgenic plants showed about 40% higher tolerance than non-transgenic or empty vector-transformed plants.-
dc.publisherKorea Soc-Assoc-Inst-
dc.titleSelection of transgenic sweetpotato plants expressing 2-Cys peroxiredoxin with enhanced tolerance to oxidative stress = Peroxiredoxin 유전자 발현 산화스트레스 내성 형질전환 고구마의 선발-
dc.title.alternativeSelection of transgenic sweetpotato plants expressing 2-Cys peroxiredoxin with enhanced tolerance to oxidative stress-
dc.typeArticle-
dc.citation.titleJournal of Plant Biotechnology-
dc.citation.number1-
dc.citation.endPage80-
dc.citation.startPage75-
dc.citation.volume36-
dc.contributor.affiliatedAuthorMyoung Duck Kim-
dc.contributor.affiliatedAuthorKyoung-Sil Yang-
dc.contributor.affiliatedAuthorSuk Yoon Kwon-
dc.contributor.affiliatedAuthorSang Soo Kwak-
dc.contributor.affiliatedAuthorHaeng Soon Lee-
dc.contributor.alternativeName김명덕-
dc.contributor.alternativeName양경실-
dc.contributor.alternativeName권석윤-
dc.contributor.alternativeName이상열-
dc.contributor.alternativeName곽상수-
dc.contributor.alternativeName이행순-
dc.identifier.bibliographicCitationJournal of Plant Biotechnology, vol. 36, no. 1, pp. 75-80-
dc.identifier.doi10.5010/JPB.2009.36.1.075-
dc.description.journalClassN-
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Division of Research on National Challenges > Plant Systems Engineering Research > 1. Journal Articles
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