High frequency plant regeneration from transverse thin cell layers in indian mustard (Brassica juncea L.)

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dc.contributor.authorM S Bhuiyan-
dc.contributor.authorY P Lim-
dc.contributor.authorSung Ran Min-
dc.contributor.authorK S Choi-
dc.contributor.authorJang Ryol Liu-
dc.date.accessioned2017-04-19T09:13:40Z-
dc.date.available2017-04-19T09:13:40Z-
dc.date.issued2009-
dc.identifier.issn1229-2818-
dc.identifier.uri10.5010/JPB.2009.36.1.081ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/8923-
dc.description.abstractAn efficient and reproducible plant regeneration system was established using transverse thin cell layers (tTCLs) in five cultivars of Brassjca juncea L. The effects of medium conditions, explant types (tTCLs of hypcotyl and cotyledonary petiole) on shoot regeneration were examined in this study. The maximum shoot regeneration frequency was obtained in Murashige and Skoog (MS) medium supplemented with 4 mg/L 6-benzylaminopurine (BA) and 0.2 mg/L 1-naphthaleneacetic acid (NAA). The hypocotyls derived tTCL explants had more shoot regeneration frequency (52%) than the cotyledonary petiole derived tTCL explants. Shoot induction was further improved by the addition of silver nitrate () in the regeneration medium. A significant genotypic effect was also observed between the five cultivars; Rai-5 displayed higher capacities to produce shoots than other cultivars. Regenerated shoots were rooted on MS basal medium without PGRs which induced 90% of roots. The plantlets established in greenhouse conditions with 99% survival, flowered normally and set seeds. The regenerated plants were fertile and identical to source plants.-
dc.publisherKorea Soc-Assoc-Inst-
dc.titleHigh frequency plant regeneration from transverse thin cell layers in indian mustard (Brassica juncea L.)-
dc.title.alternativeHigh frequency plant regeneration from transverse thin cell layers in indian mustard (Brassica juncea L.)-
dc.typeArticle-
dc.citation.titleJournal of Plant Biotechnology-
dc.citation.number1-
dc.citation.endPage86-
dc.citation.startPage81-
dc.citation.volume36-
dc.contributor.affiliatedAuthorSung Ran Min-
dc.contributor.affiliatedAuthorJang Ryol Liu-
dc.contributor.alternativeNameBhuiyan-
dc.contributor.alternativeName임용표-
dc.contributor.alternativeName민성란-
dc.contributor.alternativeName최관삼-
dc.contributor.alternativeName유장렬-
dc.identifier.bibliographicCitationJournal of Plant Biotechnology, vol. 36, no. 1, pp. 81-86-
dc.identifier.doi10.5010/JPB.2009.36.1.081-
dc.description.journalClassN-
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Division of Research on National Challenges > Plant Systems Engineering Research > 1. Journal Articles
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