Temperature of peptide ions generated by matrix-assisted laser desorption ionization and their dissociation kinetic parameters

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Title
Temperature of peptide ions generated by matrix-assisted laser desorption ionization and their dissociation kinetic parameters
Author(s)
Jeong Hee Moon; S H Yoon; M S Kim
Bibliographic Citation
Journal of Physical Chemistry B, vol. 113, no. 7, pp. 2071-2076
Publication Year
2009
Abstract
Product ion yields in postsource decay and photodissociation at 193 and 266 nm were measured for some peptide ions without a basic amino acid residue ([Y6+ H]+, [F5 + H]+, and [YPFVEPI + H]+) generated by matrix-assisted laser desorption ionization (MALDI). Data indicated statistical nature for the dissociation processes. Assuming that peptide ions formed by MALDI are in thermal equilibrium at temperature T and that their dissociation rate constants are specified by the critical energy (E0) and entropy (ΔSN), a method based on kinetic analysis was devised to determine these parameters simultaneously. The matrix used was found to affect the effective temperature of peptide ions, 2,5-dihydroxybenzoic acid (400-430 K) < sinapinic acid (440 K) < α-cyano-4-hydroxycinnamic acid (460-510 K), in agreement with previous perceptions. E0 of around 0.6 eV and ΔSN of -24 eu were smaller than previous quantum chemical results for small model peptide ions.
ISSN
1520-6106
Publisher
Amer Chem Soc
DOI
http://dx.doi.org/10.1021/jp810077e
Type
Article
Appears in Collections:
Division of Bio Technology Innovation > Core Research Facility & Analysis Center > 1. Journal Articles
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