Involvement of PTP-RQ in differentiation during adipogenesis of human mesenchymal stem cells

Cited 27 time in scopus
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Title
Involvement of PTP-RQ in differentiation during adipogenesis of human mesenchymal stem cells
Author(s)
Hyeyun Jung; Won Kon-Kim; D H Kim; Yeesook ChoSeung Jun KimSung Goo ParkByoung Chul Park; H M Lim; Kwang-Hee BaeSang Chul Lee
Bibliographic Citation
Biochemical and Biophysical Research Communications, vol. 383, no. 2, pp. 252-257
Publication Year
2009
Abstract
Mesenchymal stem cells (MSCs) are self-renewable multipotent progenitor cells with the capacity to differentiate into several distinct mesenchymal lineages. While MSCs display significant potential in tissue engineering and therapeutic applications, the regulatory mechanisms underlying the differentiation of these cells are yet to be established. Phosphorylation is a post-translational modification that plays a significant role in diverse biological phenomena. In this study, to mine the protein tyrosine phosphatases (PTPs) involved in adipogenesis of human MSCs, differential expression of human PTPs was examined using RT-PCR analysis. Among the 107 human PTPs, PTP-RQ was dramatically downregulated during the early phase of adipogenesis. PTP-RQ is classified as a receptor-type III PTP with phosphatidylinositol phosphatase (PIPase) activity. Overexpression of PTP-RQ consistently led to reduced differentiation of MSCs into adipocytes via decreasing the phosphatidyl inositol phosphate level in cells, and consequently downregulating Akt/PKB phosphorylation. Our results collectively suggest that PTP-RQ is a useful target protein for regulating the differentiation of MSCs into adipocytes, and may be used to develop novel drugs for the treatment of obesity.
Keyword
adipocyteadipogenesisdifferentiationhuman mesenchymal stem cellsprotein tyrosine phosphatase RQ
ISSN
0006-291X
Publisher
Elsevier
DOI
http://dx.doi.org/10.1016/j.bbrc.2009.04.001
Type
Article
Appears in Collections:
Division of Biomedical Research > Metabolic Regulation Research Center > 1. Journal Articles
Division of Biomedical Research > Immunotherapy Research Center > 1. Journal Articles
Critical Diseases Diagnostics Convergence Research Center > 1. Journal Articles
Division of Biomedical Research > Disease Target Structure Research Center > 1. Journal Articles
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