Induction of DNA damage in L5178Y cells treated with gold nanoparticle

Cited 20 time in scopus
Metadata Downloads

Full metadata record

DC FieldValueLanguage
dc.contributor.authorJ S Kang-
dc.contributor.authorY N Yum-
dc.contributor.authorJ H Kim-
dc.contributor.authorH Song-
dc.contributor.authorJin Young Jeong-
dc.contributor.authorYong Taik Lim-
dc.contributor.authorBong Hyun Chung-
dc.contributor.authorS N Park-
dc.date.accessioned2017-04-19T09:13:41Z-
dc.date.available2017-04-19T09:13:41Z-
dc.date.issued2009-
dc.identifier.issn1976-9148-
dc.identifier.uri10.4062/biomolther.2009.17.1.92ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/8931-
dc.description.abstractAs nanomaterials might enter into cells and have high reactivity with intracellular structures, it is necessary to assay possible genotoxic risk of them. One of these approaches, we investigated possible genotoxic potential of gold nanoparticle (AuNP) using L5178Y cells. Four different sizes of AuNP (4, 15, 100 or 200 nm) were synthesized and the sizes and structures of AuNP were analyzed using transmission electron microscopy (TEM), scanning electron microscopy (SEM) and stability was analyzed by a UV/Vis. Spectrophotometer. Cytotoxicity was assessed by direct cell counting, and cellular location was detected by dark field microscope at 6, 24 and 48 h after treatment of AuNP. Comet assay was conducted to examine DNA damage and tumor necrosis factor (TNF)-α mRNA level was assay by real-time reverse transcription polymerase chain reaction. Synthetic AuNP (4, 50, 100 and 200 nm size) had constant characteristics and stability confirmed by TEM, SEM and spectrophotometer for 10 days, respectively. Dark field microscope revealed the location of AuNP in the cytoplasm at 6, 24 and 48 h. Treatment of 4 nm AuNP induced dose and time dependent cytotoxicity, while other sizes of AuNP did not. However, Comet assay represented that treatment of 100 nm and 200 nm AuNP significantly increased DNA damage compared to vehicle control (p <0.01). Treatment of 100 nm and 200 nm AuNP significantly increased TNF-α mRNA expression compared to vehicle control (p<0.05, p<0.01, respectively). Taken together, AuNP induced DNA damage in L5178Y cell, associated with induction of oxidative stress.-
dc.publisherKorea Soc-Assoc-Inst-
dc.titleInduction of DNA damage in L5178Y cells treated with gold nanoparticle-
dc.title.alternativeInduction of DNA damage in L5178Y cells treated with gold nanoparticle-
dc.typeArticle-
dc.citation.titleBiomolecules & Therapeutics-
dc.citation.number1-
dc.citation.endPage97-
dc.citation.startPage92-
dc.citation.volume17-
dc.contributor.affiliatedAuthorJin Young Jeong-
dc.contributor.affiliatedAuthorYong Taik Lim-
dc.contributor.affiliatedAuthorBong Hyun Chung-
dc.contributor.alternativeName강진석-
dc.contributor.alternativeName염영나-
dc.contributor.alternativeName김주환-
dc.contributor.alternativeName송현아-
dc.contributor.alternativeName정진영-
dc.contributor.alternativeName임용택-
dc.contributor.alternativeName정봉현-
dc.contributor.alternativeName박순희-
dc.identifier.bibliographicCitationBiomolecules & Therapeutics, vol. 17, no. 1, pp. 92-97-
dc.identifier.doi10.4062/biomolther.2009.17.1.92-
dc.subject.keywordgenetic toxicity-
dc.subject.keywordgold nanoparticle-
dc.subject.keywordL5178Y cell-
dc.subject.keywordDNA damage-
dc.subject.localgenetic toxicity-
dc.subject.localgold nanoparticle-
dc.subject.localGold nanoparticles-
dc.subject.localGold nanoparticle (AuNP)-
dc.subject.localGold nanoparticle-
dc.subject.localL5178Y cell-
dc.subject.localDNA damage-
dc.description.journalClassY-
Appears in Collections:
Division of Research on National Challenges > Environmental diseases research center > 1. Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.