Monitoring bacterial population dynamics using real-time PCR during the bioremediation of crude-oil-contaminated soil

Cited 24 time in scopus
Metadata Downloads
Title
Monitoring bacterial population dynamics using real-time PCR during the bioremediation of crude-oil-contaminated soil
Author(s)
Geang Hea Beak; Byung Dae Yoon; Dae Hyun Cho; Byung-Hyuk Kim; Hee-Mock OhHee-Sik Kim
Bibliographic Citation
Journal of Microbiology and Biotechnology, vol. 19, no. 4, pp. 339-345
Publication Year
2009
Abstract
We evaluated the activity and abundance of the crudeoil- degrading bacterium Nocardia sp. H17-1 during bioremediation of oil-contaminated soil, using real-time PCR. The total petroleum hydrocarbon (TPH) degradation rate constants (k) of the soils treated with and without H17-1 were 0.103 d-1 and 0.028 d-1, respectively. The degradation rate constant was 3.6 times higher in the soil with H17-1 than in the soil without H17-1. In order to detect and quantify the Nocardia sp. H17-1 in soil samples, we quantified the genes encoding 16S ribosomal RNA (16S rRNA), alkane monooxygenase (alkB4), and catechol 2,3-dioxygenase (23CAT) with real-time PCR using SYBR green. The amounts of H17-1 16S rRNA and alkB4 detected increased rapidly up to 1,000-folds for the first 10 days, and then continued to increase only slightly or leveled off. However, the abundance of the 23CAT gene detected in H17-1-treated soil, where H17-1 had neither the 23CAT gene for the degradation of aromatic hydrocarbons nor the catechol 2,3-dioxygenase activity, did not differ significantly from that of the untreated soil (alpha=0.05, p>0.22).
Keyword
BioaugmentationCrude oilNocardia spReal-time PCRTotal petroleum hydrocarbon
ISSN
1017-7825
Publisher
Korea Soc-Assoc-Inst
DOI
http://dx.doi.org/10.4014/jmb.0807.423
Type
Article
Appears in Collections:
Synthetic Biology and Bioengineering Research Institute > Cell Factory Research Center > 1. Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.