Cloning of a gene encoding dextranase from Lipomyces starkeyi and its expression in Pichia pastoris

Cited 9 time in scopus
Metadata Downloads

Full metadata record

DC FieldValueLanguage
dc.contributor.authorH K Kang-
dc.contributor.authorJi Young Park-
dc.contributor.authorJ S Ahn-
dc.contributor.authorS H Kim-
dc.contributor.authorD Kim-
dc.date.accessioned2017-04-19T09:13:43Z-
dc.date.available2017-04-19T09:13:43Z-
dc.date.issued2009-
dc.identifier.issn1017-7825-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/8941-
dc.description.abstractA gene (lsd1) encoding dextranase from Lipomyces starkeyi KSM22 has been previously cloned, sequenced, and expressed in Saccharomyces cerevisiae. The gene consisting of 1,824 base pairs and encoding a protein of 608 amino acids was then cloned into and secretively expressed in Pichia pastoris under the control of the AOX1 promoter. The dextranase productivity of the P. pastoris transformant (pPIC9K-LSD1, 134,000 U/l) was approximately 4.2-fold higher than that of the S. cerevisiae transformant (pYLSD1, 32,000 U/l) cultured in an 8-1 fermentor. Over 0.63 g/l of active dextranase was secreted into the medium after methanol induction. The dextranase of the P. pastoris transformant, as analyzed by SDS-PAGE and Western blotting, showed only one homogeneous band. This dextranase of the P. pastoris transformant showed a broad band near 73 kDa. Rabbit monoclonal antibodies against a synthetic LSD1 peptide mix also recognized approximately 73 kDa.-
dc.publisherKorea Soc-Assoc-Inst-
dc.titleCloning of a gene encoding dextranase from Lipomyces starkeyi and its expression in Pichia pastoris-
dc.title.alternativeCloning of a gene encoding dextranase from Lipomyces starkeyi and its expression in Pichia pastoris-
dc.typeArticle-
dc.citation.titleJournal of Microbiology and Biotechnology-
dc.citation.number2-
dc.citation.endPage177-
dc.citation.startPage172-
dc.citation.volume19-
dc.contributor.affiliatedAuthorJi Young Park-
dc.contributor.alternativeName강희경-
dc.contributor.alternativeName박지영-
dc.contributor.alternativeName안준섭-
dc.contributor.alternativeName김승혁-
dc.contributor.alternativeName김도만-
dc.identifier.bibliographicCitationJournal of Microbiology and Biotechnology, vol. 19, no. 2, pp. 172-177-
dc.identifier.doi10.4014/jmb.0802.100-
dc.subject.keyworddextran-
dc.subject.keyworddextranase-
dc.subject.keywordexpression-
dc.subject.keywordlipomyces starkeyi-
dc.subject.keywordpichia pastoris-
dc.subject.localDextran-
dc.subject.localdextran-
dc.subject.localdextranase-
dc.subject.localDextranase-
dc.subject.localexpression-
dc.subject.localExpression-
dc.subject.locallipomyces starkeyi-
dc.subject.localpichia pastoris-
dc.subject.localPichia pastoris-
dc.description.journalClassY-
Appears in Collections:
Jeonbuk Branch Institute > Functional Biomaterial Research Center > 1. Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.