Importance of the porcine ADAM3 disintegrin domain in sperm-egg interaction = 돼지 ADAM3 disintegrin domain이 정자와 난자의 결합에 중요

Cited 6 time in scopus
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Title
Importance of the porcine ADAM3 disintegrin domain in sperm-egg interaction = 돼지 ADAM3 disintegrin domain이 정자와 난자의 결합에 중요
Author(s)
Ekyune Kim; K E Park; Ji Su Kim; Dong Chul Baek; J W Lee; Sang Rae Lee; Myeong Su Kim; Sang-Hyun Kim; C S Kim; D B Koo; H S Kang; Z Y Ryoo; Kyu Tae Chang
Bibliographic Citation
Journal of Reproduction and Development, vol. 55, no. 2, pp. 156-162
Publication Year
2009
Abstract
In the mouse, ADAM3, a well-characterized testis-specific protein of the A disintegrin and metalloprotease (ADAM) family, has a crucial role in fertilization by mediating sperm binding to the egg zona pellucida. However, little is known about ADAM3 in other species, such as domestic pigs. We have identified porcine ADAM3 and analyzed the protein. RT-PCR and trypsinization of sperm surface proteins revealed that porcine ADAM3 is expressed at high levels in the testis and on the sperm surface. Furthermore, an IVF inhibition assay with a recombinant porcine ADAM3 disintegrin domain showed that treatment of the disintegrin domain effectively prevented pig sperm-egg interactions. In the present study, we demonstrated the presence of ADAM3a and ADAM3b molecules in the pig and examined their roles in fertilization.
Keyword
A disintegrin and metalloprotease (ADAM)Disintegrin domainPorcineSperm-egg interaction
ISSN
I000-0068
Publisher
Society Reproduction & Development-Srd
DOI
http://dx.doi.org/10.1262/jrd.20134
Type
Article
Appears in Collections:
Jeonbuk Branch Institute > Primate Resources Center > 1. Journal Articles
Ochang Branch Institute > Division of National Bio-Infrastructure > National Primate Research Center > 1. Journal Articles
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