Identification of polymyxin synthetase gene cluster of Paenibacillus polymyxa and heterologous expression of the gene in Bacillus subtilis = 패니바실러스 폴리믹사균의 폴리믹신 생합성유전자군 동정 및 고초균에서의 발현

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Title
Identification of polymyxin synthetase gene cluster of Paenibacillus polymyxa and heterologous expression of the gene in Bacillus subtilis = 패니바실러스 폴리믹사균의 폴리믹신 생합성유전자군 동정 및 고초균에서의 발현
Author(s)
Soo Keun Choi; Soo Young Park; R Kim; Seong-Bin Kim; C H Lee; Jihyun Kim; Seung Hwan Park
Bibliographic Citation
Journal of Bacteriology, vol. 191, no. 10, pp. 3350-3358
Publication Year
2009
Abstract
Polymyxin, a long-known peptide antibiotic, has recently been reintroduced in clinical practice because it is sometimes the only available antibiotic for the treatment of multidrug-resistant gram-negative pathogenic bacteria. Lack of information on the biosynthetic genes of polymyxin, however, has limited the study of structure-function relationships and the development of improved polymyxins. During whole genome sequencing of Paenibacillus polymyxa E681, a plant growth-promoting rhizobacterium, we identified a gene cluster encoding polymyxin synthetase. Here, we report the complete sequence of the gene cluster and its function in polymyxin biosynthesis. The gene cluster spanning the 40.6-kb region consists of five open reading frames, designated pmxA, pmxB, pmxC, pmxD, and pmxE. The pmxC and pmxD genes are similar to genes that encode transport proteins, while pmxA, pmxB, and pmxE encode polymyxin synthetases. The insertional disruption of pmxE led to a loss of the ability to produce polymyxin. Introduction of the pmx gene cluster into the amyE locus of the Bacillus subtilis chromosome resulted in the production of polymyxin in the presence of extracellularly added L-2,4-diaminobutyric acid. Taken together, our findings demonstrate that the pmx gene cluster is responsible for polymyxin biosynthesis.
ISSN
0021-9193
Publisher
Amer Soc Microb
DOI
http://dx.doi.org/10.1128/JB.01728-08
Type
Article
Appears in Collections:
Division of Research on National Challenges > Infectious Disease Research Center > 1. Journal Articles
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