DC Field | Value | Language |
---|---|---|
dc.contributor.author | Jeong Woo Seo | - |
dc.contributor.author | Won-Kyung Hong | - |
dc.contributor.author | D Rairakhwada | - |
dc.contributor.author | Pil-Soo Seo | - |
dc.contributor.author | Min-Ho Choi | - |
dc.contributor.author | K B Song | - |
dc.contributor.author | Sang Ki Rhee | - |
dc.contributor.author | Chul Ho Kim | - |
dc.date.accessioned | 2017-04-19T09:13:46Z | - |
dc.date.available | 2017-04-19T09:13:46Z | - |
dc.date.issued | 2009 | - |
dc.identifier.issn | 0141-5492 | - |
dc.identifier.uri | 10.1007/s10529-009-9941-4 | ko |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/8951 | - |
dc.description.abstract | The levansucrase gene (lsrA) from Rahnella aquatilis was strongly expressed in a constitutive manner in Escherichia coli when cloned into a pBluescript KS-based pRL1CP plasmid vector. The native promoter upstream of lsrA and the lacZ promoter cooperatively enhanced the expression of lsrA to a level that was comparable to that of the T7 promoter, which is used in commercial pET expression vector system. A putative rho-independent transcription termination signal downstream of lsrA was crucial for gene expression. This plasmid vector also proved to be applicable for efficient expression of other foreign genes in E. coli. | - |
dc.publisher | Springer | - |
dc.title | An efficient plasmid vector for constitutive high-level expression of foreign genes in Escherichia coli = 대장균에서 외래유전자를 항시 고발현 시키기 위한 발현 벡터 | - |
dc.title.alternative | An efficient plasmid vector for constitutive high-level expression of foreign genes in Escherichia coli | - |
dc.type | Article | - |
dc.citation.title | Biotechnology Letters | - |
dc.citation.number | 6 | - |
dc.citation.endPage | 881 | - |
dc.citation.startPage | 877 | - |
dc.citation.volume | 31 | - |
dc.contributor.affiliatedAuthor | Jeong Woo Seo | - |
dc.contributor.affiliatedAuthor | Won-Kyung Hong | - |
dc.contributor.affiliatedAuthor | Min-Ho Choi | - |
dc.contributor.affiliatedAuthor | Sang Ki Rhee | - |
dc.contributor.affiliatedAuthor | Chul Ho Kim | - |
dc.contributor.alternativeName | 서정우 | - |
dc.contributor.alternativeName | 홍원경 | - |
dc.contributor.alternativeName | Rairakhwada | - |
dc.contributor.alternativeName | 서필수 | - |
dc.contributor.alternativeName | 최민호 | - |
dc.contributor.alternativeName | 송기방 | - |
dc.contributor.alternativeName | 이상기 | - |
dc.contributor.alternativeName | 김철호 | - |
dc.identifier.bibliographicCitation | Biotechnology Letters, vol. 31, no. 6, pp. 877-881 | - |
dc.identifier.doi | 10.1007/s10529-009-9941-4 | - |
dc.subject.keyword | escherichia coli | - |
dc.subject.keyword | gene expression | - |
dc.subject.keyword | levansucrase gene | - |
dc.subject.keyword | promoter | - |
dc.subject.keyword | rahnella aquatilis | - |
dc.subject.local | Escherichia coli. | - |
dc.subject.local | escherichia coli | - |
dc.subject.local | Escherichia Coli | - |
dc.subject.local | Escherichia coli | - |
dc.subject.local | E.coli | - |
dc.subject.local | escherichia coil | - |
dc.subject.local | E. coli | - |
dc.subject.local | E. Coli | - |
dc.subject.local | Gene Expression | - |
dc.subject.local | Gene expression | - |
dc.subject.local | gene expression | - |
dc.subject.local | levansucrase gene | - |
dc.subject.local | levansucrase gene (lsrA) | - |
dc.subject.local | Promoter | - |
dc.subject.local | promoter | - |
dc.subject.local | Promoters | - |
dc.subject.local | Rahnella aquatilis | - |
dc.subject.local | rahnella aquatilis | - |
dc.description.journalClass | Y | - |
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