Assessment of gene flow from genetically modified anthracnose-resistant chili pepper (Capsicum annuum L.) to a conventional crop = 유전자변형고추의 유전자이동 평가

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Title
Assessment of gene flow from genetically modified anthracnose-resistant chili pepper (Capsicum annuum L.) to a conventional crop = 유전자변형고추의 유전자이동 평가
Author(s)
Chang-Gi Kim; Dae In Kim; Hyo Jeong Kim; Ji Eun Park; B Lee; Kee Woong Park; Soon Chun Jeong; Kyung Hwa Choi; J H An; K H Cho; Y S Kim; Hwan Mook Kim
Bibliographic Citation
Journal of Plant Biology, vol. 52, no. 3, pp. 251-258
Publication Year
2009
Abstract
We conducted a 2-year field assessment of the gene flow from genetically modified (GM) chili pepper (Capsicum annuum L.), containing the PepEST (pepper esterase) gene, to a non-GM control line "WT512" and two commercial hybrid cultivars, "Manidda" and "Cheongpung Myeongwol (CM)." After seeds were collected from the pollen-recipient non-GM plants, hybrids between them and the GM peppers were screened by a hygromycin assay. PCR with the targeting hpt gene was performed to confirm the presence of transgenes in hygromycin-resistant seedlings. Out of 7,071 "WT512" seeds and 6,854 "Manidda" seeds collected in 2006, eight and 12 hybrids, respectively, were detected. In 2007, 33 hybrids from 3,456 "WT512" seeds and 50 hybrids from 3,457 "CM" seeds were found. The highest frequency of gene flow, 6.19%, was observed in that 2007 trial. These results suggest that a limited isolation distance would be sufficient to prevent gene flow from GM to conventionally bred chili peppers.
Keyword
chili peppergene flowgenetically modified (GM) cropcapsicum annuum
ISSN
I000-0094
Publisher
Springer
DOI
http://dx.doi.org/10.1007/s12374-009-9025-y
Type
Article
Appears in Collections:
Division of Bio Technology Innovation > Bio-Evaluation Center > 1. Journal Articles
National Research Safety Headquarter > 1. Journal Articles
Ochang Branch Institute > 1. Journal Articles
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