Upregulation of the cycline kinase subunit CKS2 increases cell proliferation rate in gastric cancer

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Title
Upregulation of the cycline kinase subunit CKS2 increases cell proliferation rate in gastric cancer
Author(s)
Min Ah Kang; Jong-Tae Kim; J H Kim; S Y Kim; Y H Kim; Young Il Yeom; Y Lee; Hee Gu Lee
Bibliographic Citation
Journal of Cancer Research and Clinical Oncology, vol. 135, no. 6, pp. 761-769
Publication Year
2009
Abstract
Purpose: CKS2 was identified as an upregulated gene in gastric cancer via our DNA microarray. This study was to verify the upregulation of CKS2 in many gastric cancer patients and to examine the CKS2-mediated cellular response. Methods: CKS2 upregulation was analyzed using reverse transcriptase PCR, real-time PCR, and immunohistochemical and clinicopathological analyses. GFP-CKS2 or CKS2-siRNA was used to analyze the cellular localization and proliferation. Results: The strong upregulation of mRNA and protein levels of CKS2 was identified. In CKS2-overexpressing cells, tumor suppressor p53 and p21cip1 were downregulated and cell growth was increased. In contrast, CKS2-siRNA-transfected cells showed an increased tumor suppressor expression and decreased cell growth. Conclusions: We showed that CKS2 was significantly upregulated in gastric cancers and a high level of CKS2 was highly correlated with histologic tumor differentiation and pathological grade of the tumor size, lymph node, and metastasis stage. We suggest that the cell cycle regulator CKS2 might be deeply involved in gastric cancer progression.
Keyword
CDK1CKS2gastric cancerp53
ISSN
0171-5216
Publisher
Springer
DOI
http://dx.doi.org/10.1007/s00432-008-0510-3
Type
Article
Appears in Collections:
Division of Biomedical Research > Immunotherapy Research Center > 1. Journal Articles
Division of Biomedical Research > Personalized Genomic Medicine Research Center > 1. Journal Articles
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