Characterization of full-length enriched expressed sequence tags of dehydration-treated white fibrous roots of sweetpotato

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Title
Characterization of full-length enriched expressed sequence tags of dehydration-treated white fibrous roots of sweetpotato
Author(s)
Sun Hyung Kim; W K Song; Yun Hee Kim; Suk Yoon Kwon; Haeng Soon Lee; I C Lee; Sang Soo Kwak
Bibliographic Citation
BMB Reports, vol. 42, no. 5, pp. 271-276
Publication Year
2009
Abstract
Sweetpotato (Ipomoea batatas (L). Lam.) is relatively tolerant to unfavorable growth conditions such as drought, yet has not been exploited to provide a better understanding of the molecular basis of drought stress tolerance. We obtained 983 high-quality expressed sequence tags of 100 bp or longer (average length of 700 bp) from cDNA libraries of detached white fibrous root tissues by subjecting them to dehydration for 6 h. The 431 cDNAs were each assigned a function by alignment using the BLASTX algorithm. Among them, three genes associated with various abiotic stresses and nine genes not previously associated with drought stress were selected for expression pattern analysis through detailed reverse transcription-polymerase chain reaction. The direct and indirect relationships of the 12 genes with drought tolerance mechanisms were ascertained at different developmental stages and under various stress conditions.
Keyword
Abiotic stressDrought stressExpressed sequence tagRootSweetpotato
ISSN
1225-8687
Publisher
Korea Soc-Assoc-Inst
DOI
http://dx.doi.org/10.5483/BMBRep.2009.42.5.271
Type
Article
Appears in Collections:
Division of Research on National Challenges > Plant Systems Engineering Research > 1. Journal Articles
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