Analysis of β-(1→3)(1→6)-glucan produced by Aureobasidium pullulans IMS-822 = Aureobasidium pullulans IMS-822가 생산하는 β-(1→3)(1→6)-glucan의 특성 분석

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Title
Analysis of β-(1→3)(1→6)-glucan produced by Aureobasidium pullulans IMS-822 = Aureobasidium pullulans IMS-822가 생산하는 β-(1→3)(1→6)-glucan의 특성 분석
Author(s)
Seog June Lee; K H AhnChan Sun Park; Byung Dae Yoon; Min-Soo Kim
Bibliographic Citation
Korean Journal of Microbiology, vol. 45, no. 1, pp. 63-68
Publication Year
2009
Abstract
The exo-polysaccharide producing microorganism, Aureobasidium pullulans IMS-822, was isolated and identified from soil. The viscosity-average molecular weight of exo-polysaccharide was calculated as 8.9×105 by Mark-Houwink equation. The sugar component of exo-polysaccharide was determined as glucose by HPLC analysis. The IR spectra indicated that the exo-polysaccharide has an absorption peak at 890 cm-1 for the β-configuration of D-glucan. The 13C NMR signal at δ 86.62 ppm arose from the substituted C-3 of glucose. The signal at δ 72.11 ppm was assigned to C-6 of branched β-(1→3)-D-glucosyl residues. Viscosity and Congo red reaction indicated that β-(1→3)(1→6)-glucan produced by A. pullulans IMS-822 has a highly ordered hydrogen-bond dependent conformation in aqueous solution, which collapses in strong alkaline solution.
Keyword
β-(1→3)(1→6)-glucanaqueous conformationaureobasidium sp.viscosity-average molecular weight
ISSN
0440-2413
Publisher
South Korea
Type
Article
Appears in Collections:
Jeonbuk Branch Institute > Functional Biomaterial Research Center > 1. Journal Articles
Jeonbuk Branch Institute > Immunoregulatory materials Research Center > 1. Journal Articles
Jeonbuk Branch Institute > Microbial Biotechnology Research Center > 1. Journal Articles
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