Spore display using Bacillus thuringiensis exosporium protein InhA

Cited 14 time in scopus
Metadata Downloads
Title
Spore display using Bacillus thuringiensis exosporium protein InhA
Author(s)
T J Park; Soo Keun ChoiHeung Chae Jung; S Y Lee; Jae Gu Pan
Bibliographic Citation
Journal of Microbiology and Biotechnology, vol. 19, no. 5, pp. 495-501
Publication Year
2009
Abstract
A new spore display method is presented that enables recombinant proteins to be displayed on the surface of Bacillus spores via fusion with InhA, an exosporium component of Bacillus thuringiensis. The green fluorescent protein and beta-galactosidase as model proteins were fused to the Cterminal region of InhA, respectively. The surface expression of the proteins on the spores was confirmed by flow cytometry, confocal laser scanning microscopy, measurement of the enzyme activity, and an immunogold electron microscopy analysis. InhA-mediated anchoring of foreign proteins in the exosporium of Bacillus spores can provide a new method of microbial display, thereby broadening the potential for novel applications of microbial display.
Keyword
Bacillus thuringiensisspore displayexosporiumInhA
ISSN
1017-7825
Publisher
Korea Soc-Assoc-Inst
DOI
http://dx.doi.org/10.4014/jmb.0802.163
Type
Article
Appears in Collections:
Division of Research on National Challenges > Infectious Disease Research Center > 1. Journal Articles
Division of Bio Technology Innovation > SME Support Center > 1. Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.