High-level production of lycopene in metabolically engineered E. coli

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dc.contributor.authorS W Kim-
dc.contributor.authorJ B Kim-
dc.contributor.authorJ M Ryu-
dc.contributor.authorJoon Ki Jung-
dc.contributor.authorJ H Kim-
dc.date.accessioned2017-04-19T09:13:52Z-
dc.date.available2017-04-19T09:13:52Z-
dc.date.issued2009-
dc.identifier.issn0032-9592-
dc.identifier.uri10.1016/j.procbio.2009.04.018ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/8979-
dc.description.abstractRecombinant lycopene was generated by utilizing metabolically engineered Escherichia coli with yields being dependent upon inocula state. Yields were especially low in the case of cultures harboring high-copy plasmids that were established with inocula at the stationary growth phase. On the other hand, cultures derived using low-copy plasmid, however, yielded high amounts of lycopene irrespective of inocula state. Nevertheless, it showed still an inocula dependence pattern in lycopene productivity (mg/l/h). To further increase lycopene productivity, we applied a temperature-shift culture technique (37 → 25 °C). Using this method, we effectively enhanced lycopene productivity without any problematic phenomena. As a result, we were able to increase lycopene yield by approximately 20% compared to previous culture methods. In the present study, we were able to reach a final lycopene yield up to 260 mg/l for 60 h, which corresponds to the highest titer to date for the production of lycopene in E. coli.-
dc.publisherElsevier-
dc.titleHigh-level production of lycopene in metabolically engineered E. coli-
dc.title.alternativeHigh-level production of lycopene in metabolically engineered E. coli-
dc.typeArticle-
dc.citation.titleProcess Biochemistry-
dc.citation.number8-
dc.citation.endPage905-
dc.citation.startPage899-
dc.citation.volume44-
dc.contributor.affiliatedAuthorJoon Ki Jung-
dc.contributor.alternativeName김성우-
dc.contributor.alternativeName김재범-
dc.contributor.alternativeName류지명-
dc.contributor.alternativeName정준기-
dc.contributor.alternativeName김정회-
dc.identifier.bibliographicCitationProcess Biochemistry, vol. 44, no. 8, pp. 899-905-
dc.identifier.doi10.1016/j.procbio.2009.04.018-
dc.subject.keywordinocula dependence-
dc.subject.keywordlycopene-
dc.subject.keywordpantoea ananatis-
dc.subject.keywordplasmid copy number-
dc.subject.keywordsynthetic operon-
dc.subject.keywordtemperature-shift-
dc.subject.localinocula dependence-
dc.subject.locallycopene-
dc.subject.localLycopene-
dc.subject.localpantoea ananatis-
dc.subject.localPantoea ananatis-
dc.subject.localplasmid copy number-
dc.subject.localSynthetic operon-
dc.subject.localsynthetic operon-
dc.subject.localtemperature-shift-
dc.description.journalClassY-
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