DC Field | Value | Language |
---|---|---|
dc.contributor.author | S W Kim | - |
dc.contributor.author | J B Kim | - |
dc.contributor.author | J M Ryu | - |
dc.contributor.author | Joon Ki Jung | - |
dc.contributor.author | J H Kim | - |
dc.date.accessioned | 2017-04-19T09:13:52Z | - |
dc.date.available | 2017-04-19T09:13:52Z | - |
dc.date.issued | 2009 | - |
dc.identifier.issn | 0032-9592 | - |
dc.identifier.uri | 10.1016/j.procbio.2009.04.018 | ko |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/8979 | - |
dc.description.abstract | Recombinant lycopene was generated by utilizing metabolically engineered Escherichia coli with yields being dependent upon inocula state. Yields were especially low in the case of cultures harboring high-copy plasmids that were established with inocula at the stationary growth phase. On the other hand, cultures derived using low-copy plasmid, however, yielded high amounts of lycopene irrespective of inocula state. Nevertheless, it showed still an inocula dependence pattern in lycopene productivity (mg/l/h). To further increase lycopene productivity, we applied a temperature-shift culture technique (37 → 25 °C). Using this method, we effectively enhanced lycopene productivity without any problematic phenomena. As a result, we were able to increase lycopene yield by approximately 20% compared to previous culture methods. In the present study, we were able to reach a final lycopene yield up to 260 mg/l for 60 h, which corresponds to the highest titer to date for the production of lycopene in E. coli. | - |
dc.publisher | Elsevier | - |
dc.title | High-level production of lycopene in metabolically engineered E. coli | - |
dc.title.alternative | High-level production of lycopene in metabolically engineered E. coli | - |
dc.type | Article | - |
dc.citation.title | Process Biochemistry | - |
dc.citation.number | 8 | - |
dc.citation.endPage | 905 | - |
dc.citation.startPage | 899 | - |
dc.citation.volume | 44 | - |
dc.contributor.affiliatedAuthor | Joon Ki Jung | - |
dc.contributor.alternativeName | 김성우 | - |
dc.contributor.alternativeName | 김재범 | - |
dc.contributor.alternativeName | 류지명 | - |
dc.contributor.alternativeName | 정준기 | - |
dc.contributor.alternativeName | 김정회 | - |
dc.identifier.bibliographicCitation | Process Biochemistry, vol. 44, no. 8, pp. 899-905 | - |
dc.identifier.doi | 10.1016/j.procbio.2009.04.018 | - |
dc.subject.keyword | inocula dependence | - |
dc.subject.keyword | lycopene | - |
dc.subject.keyword | pantoea ananatis | - |
dc.subject.keyword | plasmid copy number | - |
dc.subject.keyword | synthetic operon | - |
dc.subject.keyword | temperature-shift | - |
dc.subject.local | inocula dependence | - |
dc.subject.local | lycopene | - |
dc.subject.local | Lycopene | - |
dc.subject.local | pantoea ananatis | - |
dc.subject.local | Pantoea ananatis | - |
dc.subject.local | plasmid copy number | - |
dc.subject.local | Synthetic operon | - |
dc.subject.local | synthetic operon | - |
dc.subject.local | temperature-shift | - |
dc.description.journalClass | Y | - |
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