Phosphate-responsive promoter of a Pichia pastoris sodium phosphate symporter

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dc.contributor.authorJungoh Ahn-
dc.contributor.authorJi-Yeon Hong-
dc.contributor.authorM Park-
dc.contributor.authorHyeok Won Lee-
dc.contributor.authorEun Gyo Lee-
dc.contributor.authorChun Sug Kim-
dc.contributor.authorJoohwan Lee-
dc.contributor.authorEui Sung Choi-
dc.contributor.authorJoon Ki Jung-
dc.contributor.authorHong-Weon Lee-
dc.date.accessioned2017-04-19T09:13:53Z-
dc.date.available2017-04-19T09:13:53Z-
dc.date.issued2009-
dc.identifier.issn0099-2240-
dc.identifier.uri10.1128/AEM.02913-08ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/8982-
dc.description.abstractTo develop a functional phosphate-regulated promoter in Pichia pastoris, a phosphate-responsive gene, PHO89, which encodes a putative sodium (Na +)-coupled phosphate symporter, was isolated. Sequencing analyses revealed a 1,731-bp open reading frame encoding a 576-amino-acid polypeptide with 12 putative transmembrane domains. The properties of the PHO89 promoter (PPHO89) were investigated using a bacterial lipase gene as a reporter in 5-liter jar fermentation experiments. PPHO89 was tightly regulated by phosphate and was highly activated when the cells were grown in a phosphate-limited external environment. Compared to translation elongation factor 1α and the glyceraldehyde-3-phosphate dehydrogenase promoter, PPHO89 exhibited strong transcriptional activity with higher specific productivity (amount of lipase produced/cell/h). Furthermore, a cost-effective and simple PPHO89-based fermentation process was developed for industrial application. These results demonstrate the potential for efficient use of PPHO89 for controlled production of recombinant proteins in P. pastoris.-
dc.publisherAmer Soc Microb-
dc.titlePhosphate-responsive promoter of a Pichia pastoris sodium phosphate symporter-
dc.title.alternativePhosphate-responsive promoter of a Pichia pastoris sodium phosphate symporter-
dc.typeArticle-
dc.citation.titleApplied and Environmental Microbiology-
dc.citation.number11-
dc.citation.endPage3534-
dc.citation.startPage3528-
dc.citation.volume75-
dc.contributor.affiliatedAuthorJungoh Ahn-
dc.contributor.affiliatedAuthorJi-Yeon Hong-
dc.contributor.affiliatedAuthorHyeok Won Lee-
dc.contributor.affiliatedAuthorEun Gyo Lee-
dc.contributor.affiliatedAuthorChun Sug Kim-
dc.contributor.affiliatedAuthorJoohwan Lee-
dc.contributor.affiliatedAuthorEui Sung Choi-
dc.contributor.affiliatedAuthorJoon Ki Jung-
dc.contributor.affiliatedAuthorHong-Weon Lee-
dc.contributor.alternativeName안정오-
dc.contributor.alternativeName홍지연-
dc.contributor.alternativeName박명수-
dc.contributor.alternativeName이혁원-
dc.contributor.alternativeName이은교-
dc.contributor.alternativeName김천석-
dc.contributor.alternativeName이주환-
dc.contributor.alternativeName최의성-
dc.contributor.alternativeName정준기-
dc.contributor.alternativeName이홍원-
dc.identifier.bibliographicCitationApplied and Environmental Microbiology, vol. 75, no. 11, pp. 3528-3534-
dc.identifier.doi10.1128/AEM.02913-08-
dc.description.journalClassY-
Appears in Collections:
Division of Bio Technology Innovation > BioProcess Engineering Center > 1. Journal Articles
Division of Bio Technology Innovation > 1. Journal Articles
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