DC Field | Value | Language |
---|---|---|
dc.contributor.author | Jungoh Ahn | - |
dc.contributor.author | Ji-Yeon Hong | - |
dc.contributor.author | M Park | - |
dc.contributor.author | Hyeok Won Lee | - |
dc.contributor.author | Eun Gyo Lee | - |
dc.contributor.author | Chun Sug Kim | - |
dc.contributor.author | Joohwan Lee | - |
dc.contributor.author | Eui Sung Choi | - |
dc.contributor.author | Joon Ki Jung | - |
dc.contributor.author | Hong-Weon Lee | - |
dc.date.accessioned | 2017-04-19T09:13:53Z | - |
dc.date.available | 2017-04-19T09:13:53Z | - |
dc.date.issued | 2009 | - |
dc.identifier.issn | 0099-2240 | - |
dc.identifier.uri | 10.1128/AEM.02913-08 | ko |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/8982 | - |
dc.description.abstract | To develop a functional phosphate-regulated promoter in Pichia pastoris, a phosphate-responsive gene, PHO89, which encodes a putative sodium (Na +)-coupled phosphate symporter, was isolated. Sequencing analyses revealed a 1,731-bp open reading frame encoding a 576-amino-acid polypeptide with 12 putative transmembrane domains. The properties of the PHO89 promoter (PPHO89) were investigated using a bacterial lipase gene as a reporter in 5-liter jar fermentation experiments. PPHO89 was tightly regulated by phosphate and was highly activated when the cells were grown in a phosphate-limited external environment. Compared to translation elongation factor 1α and the glyceraldehyde-3-phosphate dehydrogenase promoter, PPHO89 exhibited strong transcriptional activity with higher specific productivity (amount of lipase produced/cell/h). Furthermore, a cost-effective and simple PPHO89-based fermentation process was developed for industrial application. These results demonstrate the potential for efficient use of PPHO89 for controlled production of recombinant proteins in P. pastoris. | - |
dc.publisher | Amer Soc Microb | - |
dc.title | Phosphate-responsive promoter of a Pichia pastoris sodium phosphate symporter | - |
dc.title.alternative | Phosphate-responsive promoter of a Pichia pastoris sodium phosphate symporter | - |
dc.type | Article | - |
dc.citation.title | Applied and Environmental Microbiology | - |
dc.citation.number | 11 | - |
dc.citation.endPage | 3534 | - |
dc.citation.startPage | 3528 | - |
dc.citation.volume | 75 | - |
dc.contributor.affiliatedAuthor | Jungoh Ahn | - |
dc.contributor.affiliatedAuthor | Ji-Yeon Hong | - |
dc.contributor.affiliatedAuthor | Hyeok Won Lee | - |
dc.contributor.affiliatedAuthor | Eun Gyo Lee | - |
dc.contributor.affiliatedAuthor | Chun Sug Kim | - |
dc.contributor.affiliatedAuthor | Joohwan Lee | - |
dc.contributor.affiliatedAuthor | Eui Sung Choi | - |
dc.contributor.affiliatedAuthor | Joon Ki Jung | - |
dc.contributor.affiliatedAuthor | Hong-Weon Lee | - |
dc.contributor.alternativeName | 안정오 | - |
dc.contributor.alternativeName | 홍지연 | - |
dc.contributor.alternativeName | 박명수 | - |
dc.contributor.alternativeName | 이혁원 | - |
dc.contributor.alternativeName | 이은교 | - |
dc.contributor.alternativeName | 김천석 | - |
dc.contributor.alternativeName | 이주환 | - |
dc.contributor.alternativeName | 최의성 | - |
dc.contributor.alternativeName | 정준기 | - |
dc.contributor.alternativeName | 이홍원 | - |
dc.identifier.bibliographicCitation | Applied and Environmental Microbiology, vol. 75, no. 11, pp. 3528-3534 | - |
dc.identifier.doi | 10.1128/AEM.02913-08 | - |
dc.description.journalClass | Y | - |
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