Chronic glutamate toxicity in mouse cortical neuron culture

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Title
Chronic glutamate toxicity in mouse cortical neuron culture
Author(s)
Jong Seong Ha; C S Lee; J S Maeng; Ki Sun KwonSung Sup Park
Bibliographic Citation
Brain Research, vol. 1273, no. C, pp. 138-143
Publication Year
2009
Abstract
Two pathways for glutamate toxicity have been described, receptor-mediated excitotoxicity andnon-receptormediatedoxidative glutamate toxicity.Here,we showthat two distinct forms of receptor-mediated primary cortical neuronal death exist, chronic and acute glutamate toxicity, and that these depend on exposure time. In vitro, neuronal sensitivity to chronic glutamate exposure increased as neuronsmatured and the initial platingmediumcontributed as well. In immature neurons, high concentrations of glutamate induced neuronal death. The chronic glutamate toxicity was independent of neuronal density, whereas increased density potentiated acute glutamate toxicity. Activation of ionotropic glutamate receptors (iGluRs) contributed to induction of chronic and acute glutamate toxicity at similar rates at DIV14. Inactivation of the metabotropic glutamate receptors (mGluRs) by AIDA increased neuronal sensitivity to chronic glutamate exposure but not to acute exposure. Neuronal death by acute toxicity wasmuch faster than by chronic toxicity in which activation ofmGluRs was involved. These results suggest that acute glutamate toxicity is quite different from chronic toxicity, in which activation of mGluRs is associated with resistance to glutamate toxicity.
Keyword
glutamate toxicityacute exposurechronic exposureionotropic glutamate receptormetabotropic glutamate receptor
ISSN
0006-8993
Publisher
Elsevier
DOI
http://dx.doi.org/10.1016/j.brainres.2009.03.050
Type
Article
Appears in Collections:
Aging Convergence Research Center > 1. Journal Articles
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