Polyphasic taxonomy of novel actinobacteria showing macromolecule degradation potentials in bigeum Island, Korea

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Title
Polyphasic taxonomy of novel actinobacteria showing macromolecule degradation potentials in bigeum Island, Korea
Author(s)
S G Dastager; A Pandey; Jae Chan Lee; W J Li; Chang-Jin Kim
Bibliographic Citation
Current Microbiology, vol. 59, no. 1, pp. 21-29
Publication Year
2009
Abstract
Aerobic, alkaliphilic to alkalitolerant and mesophilic bacteria were isolated and characterized from soil and sediment samples collected from Bigeum Island, South Korea. The total numbers of microorganisms in the soil and sediment samples were found to be 103.105 cfu/g and 102.107 cfu/g, respectively. A total of 163 isolates were isolated and subjected to further characterization on the basis of pH, temperature and salt tolerance. Among the 163 isolates, 54 were selected based on their tolerance attributes to temperature, pH and NaCl. Out of the 54 isolates, 27 were further selected based on their multiple tolerance ability and enzyme profile and were subjected to 16S rRNA gene sequencing and phylogenetic analysis. The latter indicated that most of the Bigeum Island isolates were related to the phylum Actinobacteria. The phylogenetic tree based on 16S rRNA gene sequences placed the 27 isolates into 9 different major bacterial genera, each genus comprising pure cultures that shared B97% sequence identity and 18 putative novel species. Most of the strains were alkalitolerant and mesophilic, and produced biotechnologically important enzymes at alkaline pH.
Keyword
microbial diversitypolyphasic approachBigeum Island
ISSN
0343-8651
Publisher
Springer
DOI
http://dx.doi.org/10.1007/s00284-009-9397-8
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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