Investigation of the biological indicator for vaccine efficacy against highly pathogenic avian influenza (HPAI) H5N1 virus challenge in mice and ferrets

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dc.contributor.authorM S Song-
dc.contributor.authorT K Oh-
dc.contributor.authorP N Q Pascua-
dc.contributor.authorH J Moon-
dc.contributor.authorJ H Lee-
dc.contributor.authorY H Baek-
dc.contributor.authorK J Woo-
dc.contributor.authorY Yoon-
dc.contributor.authorM H Sung-
dc.contributor.authorHaryoung Poo-
dc.contributor.authorC J Kim-
dc.contributor.authorY K Choi-
dc.date.accessioned2017-04-19T09:14:02Z-
dc.date.available2017-04-19T09:14:02Z-
dc.date.issued2009-
dc.identifier.issn0264410X-
dc.identifier.uri10.1016/j.vaccine.2009.03.061ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/9005-
dc.description.abstractTo investigate the biological indicator for vaccine efficacy against HPAI H5N1 virus challenge of varying clades, two inactivated whole-virus H5N1 vaccines containing the hemagglutinin (HA) and neuraminidase (NA) genes of either clade 2.2 A/EM/Korea/W149/06 (RgKoreaW149/06xPR8) or clade 2.5 A/Ck/Korea/ES/03 (RgKoreaES223N/03XPR8) virus in the background of A/PR/8/34 (H1N1) were generated by reverse genetics. Administration of the vaccines (2-dose 1.77, 3.5, 7.5 or 15μg of HA) elicited high HI titers in a dose-dependent manner. Mice immunized with RgKoreaW149/06xPR8 were completely protected from challenge against wild-type A/EM/Korea/W149/06 without clinical signs of infection. RgKoreaES223N/03XPR8 could not protect mice at 1.77μg while all immunized ferrets were completely protected. Two-dose (7.5μg) vaccinated mice (HI titer ≥320) and triple dose (7.5μg) vaccinated ferrets with RgKoreaES223N/03xPR8 (HI titer ≥640) protected vaccine recipients from mortality, inhibited nasal virus shedding and limited influenza virus tropism. Thus, these vaccines provided cross-protectivity in both models. More importantly, these results collectively suggested a positive correlation between vaccine-induced HI titers and inhibition of virus shedding including block of viral proliferation in major organs against a heterologous HPAI H5N1 virus. Although developing technologies or methods that will enable the reduction of administration dose/frequency remains to be resolved, our study demonstrated a considerable biological marker (≥640 HI titer) for full protection of the vaccinated hosts that could provide a preliminary basis for the assessment of complete immunization.-
dc.publisherElsevier-
dc.titleInvestigation of the biological indicator for vaccine efficacy against highly pathogenic avian influenza (HPAI) H5N1 virus challenge in mice and ferrets-
dc.title.alternativeInvestigation of the biological indicator for vaccine efficacy against highly pathogenic avian influenza (HPAI) H5N1 virus challenge in mice and ferrets-
dc.typeArticle-
dc.citation.titleVaccine-
dc.citation.number24-
dc.citation.endPage3152-
dc.citation.startPage3145-
dc.citation.volume27-
dc.contributor.affiliatedAuthorHaryoung Poo-
dc.contributor.alternativeName송민숙-
dc.contributor.alternativeName오택규-
dc.contributor.alternativeNamePascua-
dc.contributor.alternativeName문호진-
dc.contributor.alternativeName이준한-
dc.contributor.alternativeName백윤희-
dc.contributor.alternativeName우규진-
dc.contributor.alternativeName윤엽-
dc.contributor.alternativeName성문희-
dc.contributor.alternativeName부하령-
dc.contributor.alternativeName김철중-
dc.contributor.alternativeName최영기-
dc.identifier.bibliographicCitationVaccine, vol. 27, no. 24, pp. 3145-3152-
dc.identifier.doi10.1016/j.vaccine.2009.03.061-
dc.subject.keywordHPAI H5N1-
dc.subject.keywordvaccine-
dc.subject.keywordbiological marker-
dc.subject.keywordcross-protection-
dc.subject.localHPAI H5N1-
dc.subject.localvaccine-
dc.subject.localVaccine-
dc.subject.localbiological marker-
dc.subject.localcross-protection-
dc.subject.localCross-protection-
dc.description.journalClassY-
Appears in Collections:
Division of Research on National Challenges > Infectious Disease Research Center > 1. Journal Articles
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