Analysis of trans-splicing transcripts in embryonic stem cell = 배아줄기세포에서 트랜스 스플라이싱 전사체의 분석

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Title
Analysis of trans-splicing transcripts in embryonic stem cell = 배아줄기세포에서 트랜스 스플라이싱 전사체의 분석
Author(s)
H S Ha; Jae Won HuhDae Soo KimSang Je Park; J H Bae; K Ahn; S E Yun; H S Kim
Bibliographic Citation
Journal of Life Science, vol. 19, no. 4, pp. 549-552
Publication Year
2009
Abstract
Genetic mutations by gene fusion result from chromosomal rearrangement, trans-splicing, and intergenic splicing. Trans-splicing is a phenomenon in which two pre-mRNAs grow together into one. We analyzed the trans-splicing products in embryonic stem cells. By using bioinformatic tools, 70 trans-splicing transcripts were identified. They are classified into 6 types according to fusion pattern: 5'UTR-5'UTR, 5'UTR-3'UTR, 3'UTR-3'UTR, 5'UTR-CDS, 3'UTR-CDS, CDS-CDS. The fusion products are more abundant in CDS regions than in UTR regions, which contain multiple intron numbers. Chromosome analysis showing gene fusion via trans-splicing indicated that chromosomes 17 and 19 were activated. These data are of great use for further studies in relation to fusion genes and human diseases.
Keyword
fusion genetrans-splicingbioinformaticsgenetic mutationchromosome analysis
ISSN
I000-0128
Publisher
Korea Soc-Assoc-Inst
DOI
http://dx.doi.org/10.5352/JLS.2009.19.4.549
Type
Article
Appears in Collections:
Ochang Branch Institute > Division of National Bio-Infrastructure > National Primate Research Center > 1. Journal Articles
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