Isolation and characterization of a cellulase-free endo-beta-1,4-xylanase produced by an invertebrate-symbiotic bacterium, Cellulosimicrobium sp. HY-13

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Title
Isolation and characterization of a cellulase-free endo-beta-1,4-xylanase produced by an invertebrate-symbiotic bacterium, Cellulosimicrobium sp. HY-13
Author(s)
Do Young Kim; Mi Kyoung Han; Jong Suk Lee; Hyun Woo OhDoo Sang Park; D H Shin; Kyung Sook Bae; Kwang Hee SonHo Yong Park
Bibliographic Citation
Process Biochemistry, vol. 44, no. 9, pp. 1055-1059
Publication Year
2009
Abstract
A xylanolytic bacterium, Cellulosimicrobium sp. HY-13, was isolated from the digestive tract of an earthworm, Eisenia fetida. The purified cellulase-free endo-b-1,4-xylanase (XylK) produced by strain HY-13 was found to contain an N-terminal amino acid sequence of APSTLEAAAE and to have a relative molecular mass of 36 kDa. It was most active at pH 6.0 and 55 8C and had Vmax and Km values toward oat spelt xylan of 4067 IU/mg and 2.78 mg/ml, respectively. XylK primarily degraded xylan to a series of xylooligosaccharides composed of xylobiose to xylotetraose, but it could not further hydrolyze xylobiose to xylose. The results of the present study suggest that the relatively highly active XylK lacking exoxylanolytic activity is a promising candidate for the efficient production of non-digestible xylooligosaccharides that may have beneficial effects to gastrointestinal health via promotion of the growth of probiotics.
Keyword
cellulosimicrobium sp. HY-13earthwormeisenia fetidaendo-b-1,4-xylanaseinvertebratexylooligosaccharides
ISSN
0032-9592
Publisher
Elsevier
DOI
http://dx.doi.org/10.1016/j.procbio.2009.05.005
Type
Article
Appears in Collections:
Division of Biomaterials Research > Industrial Bio-materials Research Center > 1. Journal Articles
Division of Bio Technology Innovation > Core Facility Management Center > 1. Journal Articles
Jeonbuk Branch Institute > Biological Resource Center > 1. Journal Articles
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