Upregulation of the cysteine protease inhibitor, cystatin SN, contributes to cell proliferation and cathepsin inhibition in gastric cancer

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Title
Upregulation of the cysteine protease inhibitor, cystatin SN, contributes to cell proliferation and cathepsin inhibition in gastric cancer
Author(s)
Eun Hwa Choi; Jong-Tae Kim; J H Kim; S Y Kim; Eun Young Song; Jae Wha KimSeon-Young KimYoung Il Yeom; I H Kim; Hee Gu Lee
Bibliographic Citation
Clinica Chimica Acta, vol. 406, no. 1, pp. 45-51
Publication Year
2009
Abstract
Background: Cysteine proteases like cathepsins are widely distributed proteolytic enzymes and form tight equimolar complexes with cystatins at their active sites. Among cystatins, CST1, encoding cystatin SN, is a member of the type 2 salivary cystatin family found in a variety of fluids and secretions, including plasma, tears, and saliva. CST1 was identified as an upregulated gene in gastric cancer tissues compared to noncancerous regions using our Affymetrix GeneChip microarray. Methods: The upregulation of CST1 in gastric cancer was analyzed using RT-PCR (n=15), immnohistochemistry, and clinicopathological (n=77) analysis. CST1-siRNA was used for the suppression of CST1 gene expression and cathepsin proteolytic activity was assayed. Results: CST1 was upregulated in cancerous lesions of gastric cancer tissues compared to noncancerous regions and clinicopathological analysis showed a significant correlation between high expression of CST1 and pTNM stage (p=0.044). In CST1-siRNA transfected cells, cell proliferation was reduced and the proteolytic activity of cathepsins was increased. Conclusions: CST1 might be highly involved in gastric tumorigenesis and regulate the proteolytic activity of cysteine proteases.
Keyword
CST1cystatin SNcathepsinproliferationgastric cancer
ISSN
0009-8981
Publisher
Elsevier
DOI
http://dx.doi.org/10.1016/j.cca.2009.05.008
Type
Article
Appears in Collections:
Division of Biomedical Research > Immunotherapy Research Center > 1. Journal Articles
Division of Biomaterials Research > Cell Factory Research Center > 1. Journal Articles
Korea Bioinformation Center > 1. Journal Articles
Division of Biomedical Research > Personalized Genomic Medicine Research Center > 1. Journal Articles
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