Real-time observations of intracellular Mg2+ signaling and waves in a single living ventricular myocyte cell

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Title
Real-time observations of intracellular Mg2+ signaling and waves in a single living ventricular myocyte cell
Author(s)
S Lee; Hee Gu Lee; S H Kang
Bibliographic Citation
Analytical Chemistry, vol. 81, no. 2, pp. 538-542
Publication Year
2009
Abstract
Despite the important regulatory role of Mg2+ in metabolic pathways, its underlying mechanism is not completely understood at the single-cell level. This study examined the propagation and dynamics of Mg 2+ signaling across the cell membrane by employing the real-time visualization of intracellular Mg2+ waves in living ventricular myocytes using a combination of total internal reflection fluorescence microscopy and Nomarski differential interference contrast. Real-time Mg 2+ waves and sparks in a living cell membrane were observed using a fluorescent Mg2+ indicator (mag-fluo-4-AM) in the concentration range of 5 aM-5 μM. The intracellular locations of the fluorescent Mg2+ indicator were confirmed by adding Na+ATP. The Mg2+ sparks and waves showed random temporal propagation patterns in nonhomogeneous substructures. These results show that spatiotemporal intra-cellular Mg 2+ signaling information can be obtained for individual living cells.
ISSN
0003-2700
Publisher
Amer Chem Soc
DOI
http://dx.doi.org/10.1021/ac8013324
Type
Article
Appears in Collections:
Division of Biomedical Research > Immunotherapy Research Center > 1. Journal Articles
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