Species-specific detection of Listeria monocytogenes using polymerase chain reaction assays targeting the prfA virulence gene cluster

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Title
Species-specific detection of Listeria monocytogenes using polymerase chain reaction assays targeting the prfA virulence gene cluster
Author(s)
H J Jung; S H Park; S D Ha; K H Lee; D H Chung; C H Kim; Min-Gon Kim; K Y Kim; K S Kim
Bibliographic Citation
Bioscience Biotechnology and Biochemistry, vol. 73, no. 6, pp. 1412-1415
Publication Year
2009
Abstract
Sequence comparison of individual genes in the prfA virulence gene cluster (pVGC), a central virulence gene cluster, from different Listeria species indicated that priming sites within the genes appeared to be specific for Listeria monocytogenes exclusively. Therefore, the pVGC was targeted for polymerase chain reaction(PCR) assays to detect and specifically identify L monocytogenes. Each gene of the pVGC was specifically amplified in L. monocytogenes but not in other Listeria species.
Keyword
Listeria monocytogenesPCRprfA virulence gene cluster
ISSN
0916-8451
Publisher
T&F (Taylor & Francis)
DOI
http://dx.doi.org/10.1271/bbb.70252
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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