Functional genomics of adhesion, invasion, and mycelial formation in Schizosaccharomyces pombe

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Functional genomics of adhesion, invasion, and mycelial formation in Schizosaccharomyces pombe
J Dodgson; H Avula; Kwang Lae Hoe; Dong Uk Kim; H O Park; J Hayles; J Armstrong
Bibliographic Citation
Eukaryotic Cell, vol. 8, no. 8, pp. 1298-1306
Publication Year
Investigation into the switch between single-celled and filamentous forms of fungi may provide insights into cell polarity, differentiation, and fungal pathogenicity. At the molecular level, much of this investigation has fallen on two closely related budding yeasts, Candida albicans and Saccharomyces cerevisiae. Recently, the much more distant fission yeast Schizosaccharomyces pombe was shown to form invasive filaments after nitrogen limitation (E. Amoah-Buahin, N. Bone, and J. Armstrong, Eukaryot. Cell 4:1287-1297, 2005) and this genetically tractable organism provides an alternative system for the study of dimorphic growth. Here we describe a second mode of mycelial formation of S. pombe, on rich media. Screening of an S. pombe haploid deletion library identified 12 genes required for mycelial development which encode potential transcription factors, orthologues of S. cerevisiae Sec14p and Tlg2p, and the formin For3, among others. These were further grouped into two phenotypic classes representing different stages of the process. We show that galactose-dependent cell adhesion and actin assembly are both required for mycelial formation and mutants lacking a range of genes controlling cell polarity all produce mycelia but with radically altered morphology.
American Society for Microbiology
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Division of Biomedical Research > Rare Disease Research Center > 1. Journal Articles
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