DC Field | Value | Language |
---|---|---|
dc.contributor.author | Tae Woong Whon | - |
dc.contributor.author | Y H Lee | - |
dc.contributor.author | Dong-Shan An | - |
dc.contributor.author | H K Song | - |
dc.contributor.author | Song-Gun Kim | - |
dc.date.accessioned | 2017-04-19T09:14:30Z | - |
dc.date.available | 2017-04-19T09:14:30Z | - |
dc.date.issued | 2009 | - |
dc.identifier.issn | 0021-8898 | - |
dc.identifier.uri | 10.1107/S0021889809028805 | ko |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/9097 | - |
dc.description.abstract | A simple protocol to convert sitting-drop vapor-diffusion plating into a hanging-drop vapor-diffusion experiment in protein crystallization is reported. After making a sitting-drop plate, agarose solution was added to solidify the reservoir solution, and the plates were incubated upside down. Crystallization experiments with hen egg white lysozyme, thaumatin and glucose isomerase showed that the upside-down sitting-drop method could produce single crystals with all the benefits of the hanging-drop crystallization method. | - |
dc.publisher | Int Union Crystallography | - |
dc.title | A simple technique to convert sitting-drop vapor diffusion into hanging-drop vapor diffusion by solidifying the reservoir solution with agarose = 아가로스를 이용한 새로운 결정화 방법 | - |
dc.title.alternative | A simple technique to convert sitting-drop vapor diffusion into hanging-drop vapor diffusion by solidifying the reservoir solution with agarose | - |
dc.type | Article | - |
dc.citation.title | Journal of Applied Crystallography | - |
dc.citation.number | 5 | - |
dc.citation.endPage | 976 | - |
dc.citation.startPage | 975 | - |
dc.citation.volume | 42 | - |
dc.contributor.affiliatedAuthor | Tae Woong Whon | - |
dc.contributor.affiliatedAuthor | Dong-Shan An | - |
dc.contributor.affiliatedAuthor | Song-Gun Kim | - |
dc.contributor.alternativeName | 원태웅 | - |
dc.contributor.alternativeName | 이용환 | - |
dc.contributor.alternativeName | 안동선 | - |
dc.contributor.alternativeName | 송현규 | - |
dc.contributor.alternativeName | 김성건 | - |
dc.identifier.bibliographicCitation | Journal of Applied Crystallography, vol. 42, no. 5, pp. 975-976 | - |
dc.identifier.doi | 10.1107/S0021889809028805 | - |
dc.subject.keyword | Agarose gel | - |
dc.subject.keyword | Crystallization | - |
dc.subject.keyword | Glucose isomerase | - |
dc.subject.keyword | Lysozyme | - |
dc.subject.keyword | Protein crystallography | - |
dc.subject.keyword | Solidified reservoirs | - |
dc.subject.keyword | Thaumatin | - |
dc.subject.keyword | Upside-down sitting-drop vapor diffusion | - |
dc.subject.local | Agarose gel | - |
dc.subject.local | Crystallization | - |
dc.subject.local | crystallization | - |
dc.subject.local | Glucose isomerase | - |
dc.subject.local | glucose isomerase | - |
dc.subject.local | lysozyme | - |
dc.subject.local | Lysozyme | - |
dc.subject.local | Protein crystallography | - |
dc.subject.local | Solidified reservoirs | - |
dc.subject.local | Thaumatin | - |
dc.subject.local | Upside-down sitting-drop vapor diffusion | - |
dc.description.journalClass | Y | - |
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