A simple technique to convert sitting-drop vapor diffusion into hanging-drop vapor diffusion by solidifying the reservoir solution with agarose = 아가로스를 이용한 새로운 결정화 방법

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dc.contributor.authorTae Woong Whon-
dc.contributor.authorY H Lee-
dc.contributor.authorDong-Shan An-
dc.contributor.authorH K Song-
dc.contributor.authorSong-Gun Kim-
dc.date.accessioned2017-04-19T09:14:30Z-
dc.date.available2017-04-19T09:14:30Z-
dc.date.issued2009-
dc.identifier.issn00218898-
dc.identifier.uri10.1107/S0021889809028805ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/9097-
dc.description.abstractA simple protocol to convert sitting-drop vapor-diffusion plating into a hanging-drop vapor-diffusion experiment in protein crystallization is reported. After making a sitting-drop plate, agarose solution was added to solidify the reservoir solution, and the plates were incubated upside down. Crystallization experiments with hen egg white lysozyme, thaumatin and glucose isomerase showed that the upside-down sitting-drop method could produce single crystals with all the benefits of the hanging-drop crystallization method.-
dc.publisherInt Union Crystallography-
dc.titleA simple technique to convert sitting-drop vapor diffusion into hanging-drop vapor diffusion by solidifying the reservoir solution with agarose = 아가로스를 이용한 새로운 결정화 방법-
dc.title.alternativeA simple technique to convert sitting-drop vapor diffusion into hanging-drop vapor diffusion by solidifying the reservoir solution with agarose-
dc.typeArticle-
dc.citation.titleJournal of Applied Crystallography-
dc.citation.number5-
dc.citation.endPage976-
dc.citation.startPage975-
dc.citation.volume42-
dc.contributor.affiliatedAuthorSong-Gun Kim-
dc.contributor.alternativeName원태웅-
dc.contributor.alternativeName이용환-
dc.contributor.alternativeName안동선-
dc.contributor.alternativeName송현규-
dc.contributor.alternativeName김성건-
dc.identifier.bibliographicCitationJournal of Applied Crystallography, vol. 42, no. 5, pp. 975-976-
dc.identifier.doi10.1107/S0021889809028805-
dc.subject.keywordAgarose gel-
dc.subject.keywordCrystallization-
dc.subject.keywordGlucose isomerase-
dc.subject.keywordLysozyme-
dc.subject.keywordProtein crystallography-
dc.subject.keywordSolidified reservoirs-
dc.subject.keywordThaumatin-
dc.subject.keywordUpside-down sitting-drop vapor diffusion-
dc.subject.localAgarose gel-
dc.subject.localCrystallization-
dc.subject.localGlucose isomerase-
dc.subject.localLysozyme-
dc.subject.localProtein crystallography-
dc.subject.localSolidified reservoirs-
dc.subject.localThaumatin-
dc.subject.localUpside-down sitting-drop vapor diffusion-
dc.description.journalClassY-
Appears in Collections:
Jeonbuk Branch Institute > Biological Resource Center > 1. Journal Articles
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