Cascade enzyme-linked immunosorbent assay (CELISA)

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dc.contributor.authorYoung-Mi Lee-
dc.contributor.authorY Jeong-
dc.contributor.authorH J Kang-
dc.contributor.authorSang Jeon Chung-
dc.contributor.authorBong Hyun Chung-
dc.date.accessioned2017-04-19T09:14:33Z-
dc.date.available2017-04-19T09:14:33Z-
dc.date.issued2009-
dc.identifier.issn0956-5663-
dc.identifier.uri10.1016/j.bios.2009.07.010ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/9102-
dc.description.abstractImmunoassays are representative biochemical detection methods. Among them, sandwich-type immunoassays, typified by sandwich ELISA, have used in disease diagnosis or biochemical detection with high target selectivity. Horseradish peroxidase and alkaline phosphatase have been typically used for signal amplification in ELISA. Recently developed sandwich-type immunoassays such as biobarcode immunoassays, immuno-PCR, and immuno-RCA have improved sensitivity by changing mainly the signal amplification method. To develop a novel amplification method in ELISA, an enzyme-cascading system was incorporated into an ELISA, and the new assay is termed a cascading enzyme-linked immunosorbent assay (CELISA). This CELISA includes a trypsinogen-enterokinase combination as the cascading enzyme system, and was used to detect alpha-fetoprotein (AFP), which is a liver cancer marker, and prostate-specific antigen (PSA). Using a colorimetric reagent for signal generation, CELISA had 0.1-10 pM limits-of-detection for AFP and PSA in whole human serum and assay buffers, depending on the platform, well plate, or microbead type used. This study represents the first example that incorporated an enzyme cascading step in an ELISA system, resulting in successful signal amplification with sensitive detection of pathogenic antigens in serum.-
dc.publisherElsevier-
dc.titleCascade enzyme-linked immunosorbent assay (CELISA)-
dc.title.alternativeCascade enzyme-linked immunosorbent assay (CELISA)-
dc.typeArticle-
dc.citation.titleBiosensors & Bioelectronics-
dc.citation.number2-
dc.citation.endPage337-
dc.citation.startPage332-
dc.citation.volume25-
dc.contributor.affiliatedAuthorYoung-Mi Lee-
dc.contributor.affiliatedAuthorSang Jeon Chung-
dc.contributor.affiliatedAuthorBong Hyun Chung-
dc.contributor.alternativeName이영미-
dc.contributor.alternativeName정유진-
dc.contributor.alternativeName강효진-
dc.contributor.alternativeName정상전-
dc.contributor.alternativeName정봉현-
dc.identifier.bibliographicCitationBiosensors & Bioelectronics, vol. 25, no. 2, pp. 332-337-
dc.identifier.doi10.1016/j.bios.2009.07.010-
dc.subject.keywordBiosensors-
dc.subject.keywordCELISA-
dc.subject.keywordELISA-
dc.subject.keywordEnzyme cascade-
dc.subject.keywordImmunoassays-
dc.subject.localbiosensor-
dc.subject.localBio-sensor-
dc.subject.localBiosensor-
dc.subject.localbiosensors-
dc.subject.localBiosensors-
dc.subject.localCELISA-
dc.subject.localenzyme-linked immunosorbent assay (ELISA)-
dc.subject.localEnzyme-linked immunosorbent assay(ELISA)-
dc.subject.localenzyme-linked immunosorbent assay-
dc.subject.localELISA (enzyme-liked immunosorbent assay)-
dc.subject.localELISA-
dc.subject.localEnzyme cascade-
dc.subject.localImmunoassay-
dc.subject.localImmunoassays-
dc.subject.localimmunoassay-
dc.description.journalClassY-
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