Activation of autophagy during glutamate-induced HT22 cell death

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Title
Activation of autophagy during glutamate-induced HT22 cell death
Author(s)
H Kim; Jin Sun Choi; Joohyun Ryu; Sung Goo Park; S Cho; Byoung Chul Park; D H Lee
Bibliographic Citation
Biochemical and Biophysical Research Communications, vol. 388, no. 2, pp. 339-344
Publication Year
2009
Abstract
Recent evidence suggests that autophagy plays a role in oxidative injury-induced cell death. Here we examined whether glutamate-mediated oxidative toxicity induces autophagy in murine hippocampal HT22 cells and if autophagy induction affects the molecular events associated with cell death. Markers for autophagy induction including LC3 conversion, suppression of mTOR pathway, and GFP-LC3 dot formation were enhanced by glutamate treatment. By contrast, autophagy inhibition blocked glutamate-induced LC3 conversion and consequently reduced cell death. Activation of ERK1/2, a hallmark of glutamate-induced cytotoxicity, was also decreased by autophagy inhibition. Interestingly, autophagy inhibition also affected the expression of chaperones including Hsp60 and Hsp70, which are differentially regulated during HT22 cell death. Conversely, knock-down of Hsp60 greatly decreased LC3 conversion. Together these results suggest that glutamate-induced cytotoxicity involves autophagic cell death and chaperones may play a role in this process.
Keyword
AutophagyERK1/2Glutamate toxicityHsp60HT22Molecular chaperones
ISSN
0006-291X
Publisher
Elsevier
DOI
http://dx.doi.org/10.1016/j.bbrc.2009.08.007
Type
Article
Appears in Collections:
Division of Biomedical Research > Disease Target Structure Research Center > 1. Journal Articles
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