Isolation and characterization of the eicosapentaenoic acid biosynthesis gene cluster from Shewanella sp. BR-2 = 슈와넬라속 해양미생물 유래 EPA 생합성 유전자군의 분리와 특성 규명

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Title
Isolation and characterization of the eicosapentaenoic acid biosynthesis gene cluster from Shewanella sp. BR-2 = 슈와넬라속 해양미생물 유래 EPA 생합성 유전자군의 분리와 특성 규명
Author(s)
P S Seo; Chul Ho KimOh Suk Kwon; B K Hur; Jeong Woo Seo
Bibliographic Citation
Journal of Microbiology and Biotechnology, vol. 19, no. 9, pp. 881-887
Publication Year
2009
Abstract
Forty-four eicosapentaenoic acid (EPA)-producing microbial strains were isolated from the intestines of marine fishes. Among them, one strain showing a maximum level of EPA (4.78% of total fatty acids) was identified as Shewanella sp. BR-2 on the basis of its 16S rRNA sequence. The EPA content reached a maximum level during the mid-exponential phase of cell growth, and gradually decreased with further growth of the cells. A cosmid DNA including the EPA biosynthesis gene cluster consisting of pfaA-E was isolated from a cosmid library of genomic DNA of Shewanella sp. BR-2, named pCosEPA-BR2. An E. coli clone harboring pCosEPA-BR2 produced EPA at a maximum level of 7.5% of total fatty acids, confirming the EPA biosynthesis activity of the cloned gene cluster.
Keyword
Biosynthesis gene clusterEicosapentaenoic acidPKS-like moduleShewanella sp. BR-2
ISSN
1017-7825
Publisher
Korea Soc-Assoc-Inst
DOI
http://dx.doi.org/10.4014/jmb.0902.090
Type
Article
Appears in Collections:
Jeonbuk Branch Institute > Microbial Biotechnology Research Center > 1. Journal Articles
Division of Bio Technology Innovation > SME Support Center > 1. Journal Articles
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