DC Field | Value | Language |
---|---|---|
dc.contributor.author | Seung-Wook Chi | - |
dc.contributor.author | J Kim | - |
dc.contributor.author | G S Yi | - |
dc.contributor.author | Hyo Jeong Hong | - |
dc.contributor.author | S E Ryu | - |
dc.date.accessioned | 2017-04-19T09:14:42Z | - |
dc.date.available | 2017-04-19T09:14:42Z | - |
dc.date.issued | 2009 | - |
dc.identifier.issn | 0014-5793 | - |
dc.identifier.uri | 10.1016/j.febslet.2009.08.030 | ko |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/9125 | - |
dc.description.abstract | Broadly neutralizing anti-hepatitis B virus (HBV) antibody HzKR127 undergoes a fairly large conformational change of CDR H3 loop upon binding to HBV preS1 epitope peptide. In this study, we identified low-affinity antibody-binding sites in the largely unstructured preS1 region by nuclear magnetic resonance and biochemical studies, indicating that the antibody binds to the preS1 region outside the major immune epitope with low affinity. Surface plasma resonance experiments showed that the full-length preS1 has approximately three fold higher affinity for HzKR127 Fab than the preS1 epitope peptide, suggesting that the presence of low-affinity sites in the preS1 region increases the antibody-binding affinity. Therefore, the low-affinity binding of the antibody to non-epitope regions of preS1 may contribute to effective neutralization. | - |
dc.publisher | Wiley | - |
dc.title | Broadly neutralizing anti-HBV antibody binds to non-epitope regions of preS1 | - |
dc.title.alternative | Broadly neutralizing anti-HBV antibody binds to non-epitope regions of preS1 | - |
dc.type | Article | - |
dc.citation.title | FEBS Letters | - |
dc.citation.number | 18 | - |
dc.citation.endPage | 3100 | - |
dc.citation.startPage | 3095 | - |
dc.citation.volume | 583 | - |
dc.contributor.affiliatedAuthor | Seung-Wook Chi | - |
dc.contributor.affiliatedAuthor | Hyo Jeong Hong | - |
dc.contributor.alternativeName | 지승욱 | - |
dc.contributor.alternativeName | 김진기 | - |
dc.contributor.alternativeName | 이관수 | - |
dc.contributor.alternativeName | 홍효정 | - |
dc.contributor.alternativeName | 류성언 | - |
dc.identifier.bibliographicCitation | FEBS Letters, vol. 583, no. 18, pp. 3095-3100 | - |
dc.identifier.doi | 10.1016/j.febslet.2009.08.030 | - |
dc.subject.keyword | Epitope | - |
dc.subject.keyword | Hepatitis B virus | - |
dc.subject.keyword | Neutralizing antibody | - |
dc.subject.keyword | Nuclear magnetic resonance | - |
dc.subject.keyword | preS1 | - |
dc.subject.local | Epitope | - |
dc.subject.local | epitope | - |
dc.subject.local | hepatitis B virus (HBV) | - |
dc.subject.local | Hepatitis B Virus | - |
dc.subject.local | Hepatitis B virus (HBV) | - |
dc.subject.local | hepatitis B virus | - |
dc.subject.local | hepatitis B Virus (HBV) | - |
dc.subject.local | Hepatitis B virus | - |
dc.subject.local | Neutrallizing antibody | - |
dc.subject.local | neutralizing antibody | - |
dc.subject.local | neutrallizing antibody | - |
dc.subject.local | Neutralizing antibody | - |
dc.subject.local | NMR | - |
dc.subject.local | nuclear magnetic resonance (Nmr) | - |
dc.subject.local | Nuclear magnetic resonance | - |
dc.subject.local | nuclear magnetic resonance | - |
dc.subject.local | Nuclear magnetic resonance (NMR) | - |
dc.subject.local | PreS1 | - |
dc.subject.local | preS1 | - |
dc.description.journalClass | Y | - |
There are no files associated with this item.
Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.