Comparison of several methods for effective lipid extraction from microalgae

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Title
Comparison of several methods for effective lipid extraction from microalgae
Author(s)
Jae Yon Lee; Chan Yoo; So Young Jun; Chi-Yong AhnHee-Mock Oh
Bibliographic Citation
Bioresource Technology, vol. 101, no. 1, pp. s75-s77
Publication Year
2010
Abstract
Various methods, including autoclaving, bead-beating, microwaves, sonication, and a 10% NaCl solution, were tested to identify the most effective cell disruption method. The total lipids from Botryococcus sp., Chlorella vulgaris, and Scenedesmus sp. were extracted using a mixture of chloroform and methanol (1:1). The lipid contents from the three species were 5.4-11.9, 7.9-8.1, 10.0-28.6, 6.1-8.8, and 6.8-10.9 g L-1 when using autoclaving, bead-beating, microwaves, sonication, and a 10% NaCl solution, respectively. Botryococcus sp. showed the highest oleic acid productivity at 5.7 mg L-1 d-1 when the cells were disrupted using the microwave oven method. Thus, among the tested methods, the microwave oven method was identified as the most simple, easy, and effective for lipid extraction from microalgae.
Keyword
BiodieselCell disruptionLipid extractionMicroalgae
ISSN
0960-8524
Publisher
Elsevier
DOI
http://dx.doi.org/10.1016/j.biortech.2009.03.058
Type
Article
Appears in Collections:
Synthetic Biology and Bioengineering Research Institute > Cell Factory Research Center > 1. Journal Articles
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