Evaluation of annexin II as a potential serum marker for hepatocellular carcinoma using a developed sandwich ELISA method

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Title
Evaluation of annexin II as a potential serum marker for hepatocellular carcinoma using a developed sandwich ELISA method
Author(s)
Na Young Ji; M Y Park; Yun Hee Kang; C I Lee; D G Kim; Young Il Yeom; Yeo-Jin Jang; P K Myung; J W Kim; Hee Gu LeeJae Wha Kim; Kyeong Lee; Eun Young Song
Bibliographic Citation
International Journal of Molecular Medicine, vol. 24, no. 6, pp. 765-771
Publication Year
2009
Abstract
Annexin II (Annexin A2, ANXA2) is a 36 kDa calcium-dependent phospholipid-binding protein that is located on the surface of most eukaryotic cells. ANXA2 is involved in several biological processes, including anti-inflammatory effects, Ca2+-dependent exocytosis, immune responses, Ca2+ transport and phospholipase A2 regulation. In our previous study, ANXA2 was identified as an up-regulated gene in hepatocellular carcinoma (HCC) tissue by cDNA microarray. In the present study, we have evaluated ANXA2 as a tumor-associated marker of HCC. We determined the ANXA2 levels in human liver tissues with HCC using real-time RT-PCR and Western blot analysis. For quantitative analysis of the ANXA2 protein in body fluids, we developed a sandwich ELISA system in which a polyclonal antibody and a monoclonal antibody specific to ANXA2 were employed as a capture antibody and a probe antibody, respectively. We detected the ANXA2 concentration in human serum using our newly developed system and evaluated its usefulness as a tumor marker. Overexpression of ANXA2 in human liver tissue was confirmed by real-time RT-PCR and Western blot analysis. The sandwich ELISA system for ANXA2 was developed for the detection of ANXA2 in human samples. The dose-response relationship between ANXA2 and optical density was linear in the range of 0-10 μg/ ml and the sensitivity was 0.02 μg/ml. We determined the ANXA2 concentration in serum specimens using the newly developed sandwich ELISA. The serum ANXA2 concentrations of the patients with HCC (53.38±36.23 μg/ml) were significantly elevated when compared with those of normal individuals (28.81±24.94 μg/ml). These results suggest that expression of ANXA2 may be increased in HCC patients and may play an important role in liver cancer progression. This new ELISA method can be used as a tool for the detection of ANXA2 in human serum, particularly for cancer diagnostics.
Keyword
Annexin IIELISAHepatocellular carcinomaSerumTissue
ISSN
1107-3756
Publisher
Spandidos Publ Ltd
DOI
http://dx.doi.org/10.3892/ijmm_00000290
Type
Article
Appears in Collections:
Division of Biomedical Research > Personalized Genomic Medicine Research Center > 1. Journal Articles
Division of Biomedical Research > Immunotherapy Research Center > 1. Journal Articles
Division of Biomaterials Research > Cell Factory Research Center > 1. Journal Articles
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