Clitocybins, novel isoindolinone free radical scavengers, from mushroom Clitocybe aurantiaca inhibit apoptotic cell death and cellular senescence

Cited 19 time in scopus
Metadata Downloads
Title
Clitocybins, novel isoindolinone free radical scavengers, from mushroom Clitocybe aurantiaca inhibit apoptotic cell death and cellular senescence
Author(s)
E Y Moon; J M Oh; Young-Hee Kim; In Ja Ryoo; Ick Dong Yoo
Bibliographic Citation
Biological & Pharmaceutical Bulletin, vol. 32, no. 10, pp. 1689-1694
Publication Year
2009
Abstract
High concentration of intracellular reactive oxygen species (ROS) plays a role in damaging biological systems. We isolated clitocybin A from the culture broth of Clitocybe aurantiaca and then clitocybin B and C derivatives were synthesized from clitocybin A. IMR-90 lung fibroblast cells were pre-treated or post-treated with clitocybin A, B and C to the addition of 100 μM H 2O2. These compounds inhibited the level of intracellular reactive oxygen species (ROS) and H2O2-induced cell death as judged by hypodiploid cell formation. The inhibitory effect of clitocybins on H2O2-induced cell death was comparable to that with N-acetylcysteine (NAC), a well-known ROS scavenger. The inhibition of H 2O2-induced cell death by clitocybins was mediated by the reduction of caspase 3 and 9 activation, cytochrome c release from mitochondria and the degradation of IκB- aand IκB-β, which could be resulted in the prevention of cellular senescence. It suggests that clitocybins are novel compounds scavenging ROS and protect cells from apoptosis and cellular senescence.
Keyword
ApoptosisCellular senescenceClitocybe aurantiacaClitocybinReactive oxygen species
ISSN
0918-6158
Publisher
Pharmaceutical Soc Japan
DOI
http://dx.doi.org/10.1248/bpb.32.1689
Type
Article
Appears in Collections:
Ochang Branch Institute > Chemical Biology Research Center > 1. Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.