Multi-immunogenic outer membrane vesicles derived from a MsbB-deficient Salmonella enterica serovar typhimurium mutant = MsbB 결핍 살모넬라 티피뮤리움 변이주 유래 다가면역원성 세균외막소포

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Title
Multi-immunogenic outer membrane vesicles derived from a MsbB-deficient Salmonella enterica serovar typhimurium mutant = MsbB 결핍 살모넬라 티피뮤리움 변이주 유래 다가면역원성 세균외막소포
Author(s)
Sang Rae Lee; Sang-Hyun Kim; Kang Jin Jeong; K S Kim; Young-Hyun Kim; S J Kim; Ekyune Kim; J W Kim; Kyu Tae Chang
Bibliographic Citation
Journal of Microbiology and Biotechnology, vol. 19, no. 10, pp. 1271-1279
Publication Year
2009
Abstract
To develop low endotoxic and multi-immunogenic outer membrane vesicles (OMVs), a deletion mutant of the msbB gene in Salmonella enterica serovar Typhimurium (S. Typhimurium) was used as a source of low endotoxic OMV, and an expression vector of the canine parvovirus (CPV) VP2 epitope fused to the bacterial OmpA protein was constructed and transformed into the Salmonella deltaAmsbB mutant. In a lethality test, BALB/c mice injected intraperitoneally with the Salmonella deltaAmsbB mutant survived for 7 days, whereas mice injected intraperitoneally with the wild type survived for 3 days. Moreover, all mice inoculated orally with the deltaAmsbB mutant survived for 30 days, but 80% of mice inoculated orally with the wild type survived. The OmpA::CPV VP2 epitope fusion protein was expressed successfully and associated with the outer membrane and OMV fractions from the mutant S. Typhimurium transformed with the fusion protein-expressing vector. In immunogenicity tests, sera obtained from the mice immunized with either the Salmonella msbB mutant or its OMVs containing the OmpA::CPV VP2 epitope showed bactericidal activities against wild-type S. Typhimurium and contained specific antibodies to the CPV VP2 epitope. In the hemagglutination inhibition (HI) assay as a measurement of CPV-neutralizing activity in the immune sera, there was an 8-fold increase of HI titer in the OMV-immunized group compared with the control. These results suggested that the CPV-neutralizing antibody response was raised by immunization with OMV containing the OmpA::CPV VP2 epitope, as well as the protective immune response against S. Typhimurium in BALB/c mice.
Keyword
Canine parvovirusLow endotoxicityMulti- immunogenicityOuter membrane vesicleS. Typhimurium
ISSN
1017-7825
Publisher
Korea Soc-Assoc-Inst
DOI
http://dx.doi.org/10.4014/jmb.0901.505
Type
Article
Appears in Collections:
Ochang Branch Institute > Division of National Bio-Infrastructure > National Primate Research Center > 1. Journal Articles
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