Production of lactosucrose from sucrose and lactose by a levansucrase from Zymomonas mobilis = Zymomonas mobilis 유래 레반슈크라제를 이용한 락토슈크로스 생산

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dc.contributor.authorW C Han-
dc.contributor.authorS H Byun-
dc.contributor.authorM H Kim-
dc.contributor.authorE H Sohn-
dc.contributor.authorJ D Lim-
dc.contributor.authorB H Um-
dc.contributor.authorChul Ho Kim-
dc.contributor.authorS A Kang-
dc.contributor.authorK H Jang-
dc.date.accessioned2017-04-19T09:14:57Z-
dc.date.available2017-04-19T09:14:57Z-
dc.date.issued2009-
dc.identifier.issn10177825-
dc.identifier.uri10.4014/jmb.0901.045ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/9157-
dc.description.abstractLactosucrose (4G-β-D-galactosylsucrose) is an oligosaccharide consisting of galactose, glucose, and fructose. In this study, we prepared lactosucrose from lactose and sucrose using a levansucrase derived from Zymomonas mobilis. Optimum conditions for lactosucrose formation were 23°C, pH 7.0, 18.0% (w/v) lactose monohydrate, and 18% (w/v) sucrose as substrates, and 1 unit of enzyme/ml of reaction mixture. Under these conditions, the lactosucrose conversion efficiency was 28.5%. The product was purified and confirmed to be O-β-D-galactopyranosyl-(1→4)-O-β-D- glucopyranosyl-(1→2)-β-D-fructofuranoside, or lactosucrose. A mixed-enzyme system containing a levansucrase and a glucose oxidase was applied in order to increase the efficiency of lactose and sucrose conversion to lactosucrose, which rose to 43.2% as a result.-
dc.publisherSouth Korea-
dc.titleProduction of lactosucrose from sucrose and lactose by a levansucrase from Zymomonas mobilis = Zymomonas mobilis 유래 레반슈크라제를 이용한 락토슈크로스 생산-
dc.title.alternativeProduction of lactosucrose from sucrose and lactose by a levansucrase from Zymomonas mobilis-
dc.typeArticle-
dc.citation.titleJournal of Microbiology and Biotechnology-
dc.citation.number10-
dc.citation.endPage1160-
dc.citation.startPage1153-
dc.citation.volume19-
dc.contributor.affiliatedAuthorChul Ho Kim-
dc.contributor.alternativeName한우철-
dc.contributor.alternativeName변선호-
dc.contributor.alternativeName김미현-
dc.contributor.alternativeName손은화-
dc.contributor.alternativeName임정대-
dc.contributor.alternativeName엄병헌-
dc.contributor.alternativeName김철호-
dc.contributor.alternativeName강순아-
dc.contributor.alternativeName장기효-
dc.identifier.bibliographicCitationJournal of Microbiology and Biotechnology, vol. 19, no. 10, pp. 1153-1160-
dc.identifier.doi10.4014/jmb.0901.045-
dc.subject.keywordGlucose oxidase-
dc.subject.keywordLactosucrose-
dc.subject.keywordLevansucrase-
dc.subject.keywordZymomonas mobilis-
dc.subject.localGlucose oxidase-
dc.subject.localLactosucrose-
dc.subject.localLevansucrase-
dc.subject.localZymomonas mobilis-
dc.description.journalClassY-
Appears in Collections:
Jeonbuk Branch Institute > Microbial Biotechnology Research Center > 1. Journal Articles
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