DC Field | Value | Language |
---|---|---|
dc.contributor.author | W C Han | - |
dc.contributor.author | S H Byun | - |
dc.contributor.author | M H Kim | - |
dc.contributor.author | E H Sohn | - |
dc.contributor.author | J D Lim | - |
dc.contributor.author | B H Um | - |
dc.contributor.author | Chul Ho Kim | - |
dc.contributor.author | S A Kang | - |
dc.contributor.author | K H Jang | - |
dc.date.accessioned | 2017-04-19T09:14:57Z | - |
dc.date.available | 2017-04-19T09:14:57Z | - |
dc.date.issued | 2009 | - |
dc.identifier.issn | 1017-7825 | - |
dc.identifier.uri | 10.4014/jmb.0901.045 | ko |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/9157 | - |
dc.description.abstract | Lactosucrose (4G-β-D-galactosylsucrose) is an oligosaccharide consisting of galactose, glucose, and fructose. In this study, we prepared lactosucrose from lactose and sucrose using a levansucrase derived from Zymomonas mobilis. Optimum conditions for lactosucrose formation were 23°C, pH 7.0, 18.0% (w/v) lactose monohydrate, and 18% (w/v) sucrose as substrates, and 1 unit of enzyme/ml of reaction mixture. Under these conditions, the lactosucrose conversion efficiency was 28.5%. The product was purified and confirmed to be O-β-D-galactopyranosyl-(1→4)-O-β-D- glucopyranosyl-(1→2)-β-D-fructofuranoside, or lactosucrose. A mixed-enzyme system containing a levansucrase and a glucose oxidase was applied in order to increase the efficiency of lactose and sucrose conversion to lactosucrose, which rose to 43.2% as a result. | - |
dc.publisher | Korea Soc-Assoc-Inst | - |
dc.title | Production of lactosucrose from sucrose and lactose by a levansucrase from Zymomonas mobilis = Zymomonas mobilis 유래 레반슈크라제를 이용한 락토슈크로스 생산 | - |
dc.title.alternative | Production of lactosucrose from sucrose and lactose by a levansucrase from Zymomonas mobilis | - |
dc.type | Article | - |
dc.citation.title | Journal of Microbiology and Biotechnology | - |
dc.citation.number | 10 | - |
dc.citation.endPage | 1160 | - |
dc.citation.startPage | 1153 | - |
dc.citation.volume | 19 | - |
dc.contributor.affiliatedAuthor | Chul Ho Kim | - |
dc.contributor.alternativeName | 한우철 | - |
dc.contributor.alternativeName | 변선호 | - |
dc.contributor.alternativeName | 김미현 | - |
dc.contributor.alternativeName | 손은화 | - |
dc.contributor.alternativeName | 임정대 | - |
dc.contributor.alternativeName | 엄병헌 | - |
dc.contributor.alternativeName | 김철호 | - |
dc.contributor.alternativeName | 강순아 | - |
dc.contributor.alternativeName | 장기효 | - |
dc.identifier.bibliographicCitation | Journal of Microbiology and Biotechnology, vol. 19, no. 10, pp. 1153-1160 | - |
dc.identifier.doi | 10.4014/jmb.0901.045 | - |
dc.subject.keyword | Glucose oxidase | - |
dc.subject.keyword | Lactosucrose | - |
dc.subject.keyword | Levansucrase | - |
dc.subject.keyword | Zymomonas mobilis | - |
dc.subject.local | Glucose oxidase | - |
dc.subject.local | glucose oxidase | - |
dc.subject.local | Lactosucrose | - |
dc.subject.local | Levansucrase | - |
dc.subject.local | levansucrase | - |
dc.subject.local | zymomonas mobilis | - |
dc.subject.local | ZYMOMONAS MOBILIS | - |
dc.subject.local | Zymomonas mobilis | - |
dc.description.journalClass | Y | - |
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