Acetate consumption activity directly determines the level of acetate accumulation during Escherichia coli W3110 growth

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dc.contributor.authorS Shin-
dc.contributor.authorD E Chang-
dc.contributor.authorJae Gu Pan-
dc.date.accessioned2017-04-19T09:14:59Z-
dc.date.available2017-04-19T09:14:59Z-
dc.date.issued2009-
dc.identifier.issn1017-7825-
dc.identifier.uri10.4014/jmb.0902.097ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/9167-
dc.description.abstractEscherichia coli excretes acetate during aerobic growth on glycolytic carbon sources, which has been explained as an overflow metabolism when the carbon flux into the cell exceeds the capacity of central metabolic pathways. Nonacetogenic growth of E. coli on gluconeogenic carbon sources like succinate or in carbon-limited slow growth conditions is believed an evidence for the explanation. However, we found that a strain defected in the acs (acetyl Co-A synthetase) gene, the product of which is involved in scavenging acetate, accumulated acetate even in succinate medium and in carbon-limited low growth rate condition, where as its isogenic parental strain did not. The acs promoter was inducible in noncatabolite repression condition, whereas the expression of the ackA-pta operon encoding acetate kinase and phosphotransacetylase for acetate synthesis was constitutive. Results in this study suggest that E. coli excretes and scavenges acetate simultaneously in the carbon-limited low growth condition and in nonacetogenic carbon source, and the activity of the acetate consumption pathway directly affects the accumulation level of acetate in the culture broth.-
dc.publisherKorea Soc-Assoc-Inst-
dc.titleAcetate consumption activity directly determines the level of acetate accumulation during Escherichia coli W3110 growth-
dc.title.alternativeAcetate consumption activity directly determines the level of acetate accumulation during Escherichia coli W3110 growth-
dc.typeArticle-
dc.citation.titleJournal of Microbiology and Biotechnology-
dc.citation.number10-
dc.citation.endPage1134-
dc.citation.startPage1127-
dc.citation.volume19-
dc.contributor.affiliatedAuthorJae Gu Pan-
dc.contributor.alternativeName신수안-
dc.contributor.alternativeName장동은-
dc.contributor.alternativeName반재구-
dc.identifier.bibliographicCitationJournal of Microbiology and Biotechnology, vol. 19, no. 10, pp. 1127-1134-
dc.identifier.doi10.4014/jmb.0902.097-
dc.subject.keywordAcetate kinase-
dc.subject.keywordAcetate metabolism-
dc.subject.keywordAcetyl CoA synthetase-
dc.subject.keywordGlyoxylate shunt-
dc.subject.keywordPhosphotransacetylase-
dc.subject.localAcetate kinase-
dc.subject.localAcetate metabolism-
dc.subject.localAcetyl CoA synthetase-
dc.subject.localGlyoxylate shunt-
dc.subject.localPhosphotransacetylase-
dc.subject.localphosphotransacetylase-
dc.description.journalClassY-
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