Roseomonas frigidaquae sp. nov., isolated from a water-cooling system

Abstract

A non-motile, coccobacilli-shaped, pale-pink-pigmented bacterium, designated strain CW67T, was isolated from a water-cooling system in Gwangyang, Republic of Korea. Cells were found to be Gram-negative, catalase-positive and oxidase-positive, the major fatty acids were C18:1ω7c (43.6%) and C16 : 0 (15.8%), the predominant respiratory lipoquinone was Q-10 and the DNA G+C content was 69.5 mol%. A phylogenetic tree based on 16S rRNA gene sequence comparisons showed that strain CW67T forms an evolutionary lineage within the radiation of the genus Roseomonas and that its closest relative is Roseomonas gilardii subsp. rosea MDA5605T (94.7% sequence similarity). Evidence from this polyphasic study showed that strain CW67T could not be assigned to any recognized species. It therefore represents a novel species, for which the name Roseomonas frigidaquae sp. nov. is proposed, with CW67T (5KCTC 22211T 5JCM 15073T) as the type strain.