Role of genetic factors and environmental conditions in recombinant protein production for molecular farming = 재조합 단백질의 발현을 위한 유전적, 환경적 요인의 역할

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Title
Role of genetic factors and environmental conditions in recombinant protein production for molecular farming = 재조합 단백질의 발현을 위한 유전적, 환경적 요인의 역할
Author(s)
A Jamal; K Ko; Hyun Soon Kim; Y K Choo; Hyouk Joung; K Ko
Bibliographic Citation
Biotechnology Advances, vol. 27, no. 6, pp. 914-923
Publication Year
2009
Abstract
Plants are generally considered to represent a promising heterologous expression system for the production of valuable recombinant proteins. Minimal upstream plant production cost is a salient feature driving the development of plant expression systems used for the synthesis of recombinant proteins. For such a plant expression system to be fully effective, it is first essential to improve plant productivity by plant biomass after inserting genes of interest into a suitable plant. Plant productivity is related closely to its growth and development, both of which are affected directly by environmental factors. These environmental factors that affect the cultivation conditions mainly include temperature, light, salinity, drought, nutrition, insects and pests. In addition, genetic factors that affect gene expression at the transcriptional, translational, and post-translational levels are considered to be important factors related to gene expression in plants. Thus, these factors influence both the quality and quantity of recombinant protein produced in transgenic plants. Among the genetic factors, the post-translational process is of particular interest as it influences subcellular localization, protein glycosylation, assembly and folding of therapeutic proteins, consequently affecting both protein quantity and biological quality. In this review, we discuss the effects of cultivation condition and genetic factors on recombinant protein production in transgenic plants.
Keyword
Environmental factorsGlycoproteinGlycosylationMolecular farmingRecombinant proteinTransgenic plant
ISSN
0734-9750
Publisher
Elsevier
DOI
http://dx.doi.org/10.1016/j.biotechadv.2009.07.004
Type
Article
Appears in Collections:
Division of Research on National Challenges > Plant Systems Engineering Research > 1. Journal Articles
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