Beta-glucan enhanced apoptosis in human colon cancer cells SNU-C4 = 베타글루칸이 인간결장암 암세포 SNU-C4 에서 세포괴사를 증진

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Title
Beta-glucan enhanced apoptosis in human colon cancer cells SNU-C4 = 베타글루칸이 인간결장암 암세포 SNU-C4 에서 세포괴사를 증진
Author(s)
M J Kim; S Y Hong; S K Kim; C Cheong; H J Park; H K Chun; K H Jang; Byung Dae Yoon; Chul Ho Kim; S A Kang
Bibliographic Citation
Nutrition Research and Practice, vol. 3, no. 3, pp. 180-184
Publication Year
2009
Abstract
The apoptotic effect of bacteria-derived beta-glucan was investigated in human colon cancer cells SNU-C4 using terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end labeling (TUNEL) assay, reverse transcription-polymerase chain reaction (RT-PCR) expressions of Bcl-2, Bax, and Caspase-3 genes, and assay of caspase-3 enzyme activity. beta-Glucan of 10, 50, and 100 microg/mL decreased cell viability in a dose-dependent manner with typical apoptotic characteristics, such as morphological changes of chromatin condensation and apoptotic body formation from TUNEL assay. In addition, beta-glucan (100 microg/mL) decreased the expression of Bcl-2 by 0.6 times, whereas the expression of Bax and Caspase-3 were increased by 3.1 and 2.3 times, respectively, compared to untreated control group. Furthermore, the caspase-3 activity in the beta-glucan-treated group was significantly increased compared to those in control group (P < 0.05). Bacterial derived beta-glucan could be used as an effective compound inducing apoptosis in human colon cancer.
Keyword
β-glucanapoptosiscolon cancercaspase-3
ISSN
1976-1457
Publisher
South Korea
DOI
http://dx.doi.org/10.4162/nrp.2009.3.3.180
Type
Article
Appears in Collections:
Jeonbuk Branch Institute > Microbial Biotechnology Research Center > 1. Journal Articles
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