Stable plastid transformation in Nicotiana benthamiana

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Title
Stable plastid transformation in Nicotiana benthamiana
Author(s)
S J Davarpanah; S H Jung; Y J Kim; Y I Park; Sung Ran Min; Jang Ryol Liu; Won Joong Chung
Bibliographic Citation
Journal of Plant Biology, vol. 52, no. 3, pp. 244-250
Publication Year
2009
Abstract
Plastids from Nicotiana benthamiana were transformed with the vector for dicistronic expression of two genes-aminoglycoside 3'-adenyltransferase (aadA) and green fluorescent protein (gfp)-in the plastids of Nicotiana tabacum. Transplastomic shoots exhibited green fluorescence under UV light. Transformation efficiencies were similar between species. Although the border sequence (trnI and trnA) for homologous recombination to transform the plastid genome of N. benthamiana was identical to that sequence of N. tabacum, the exception was a 9-bp addition in the intron of trnI. This indicated that the N. tabacum sequence used as a border region for recombination was sufficient to insert the foreign gene into the target site between the trnI and trnA of N. benthamiana with similar efficiency. Southern blot analysis detected the presence of aadA and gfp between trnI and trnA in the plastid genome of N. benthamiana. Northern and western blot analyses revealed high expression of gfp in the plastids from petals and leaves. Our results suggest that the plastid transformation system established here is applicable to investigations of the interactions between plastid and nucleus in N. benthamiana.
Keyword
Nicotiana benthamianaPlastid transformation
ISSN
I000-0094
Publisher
Springer
DOI
http://dx.doi.org/10.1007/s12374-009-9023-0
Type
Article
Appears in Collections:
Division of Research on National Challenges > Plant Systems Engineering Research > 1. Journal Articles
Synthetic Biology and Bioengineering Research Institute > Cell Factory Research Center > 1. Journal Articles
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