Catalytic properties of a GH10 endo-β-1,4-xylanase from Streptomyces thermocarboxydus HY-15 isolated from the gut of Eisenia fetida
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- Title
- Catalytic properties of a GH10 endo-β-1,4-xylanase from Streptomyces thermocarboxydus HY-15 isolated from the gut of Eisenia fetida
- Author(s)
- Do Young Kim; Mi Kyung Han; Hyun Woo Oh; Doo Sang Park; Su Jin Kim; Seung Goo Lee; D H Shin; Kwang Hee Son; Kyung Sook Bae; Ho Yong Park
- Bibliographic Citation
- Journal of Molecular Catalysis B: Enzymatic, vol. 62, no. 1, pp. 32-39
- Publication Year
- 2010
- Abstract
- A novel GH10 endo-β-1,4-xylanase (XylG) gene from Streptomyces thermocarboxydus HY-15, which was isolated from the gut of Eisenia fetida, was cloned, over-expressed, and characterized. The XylG gene (1182 bp) encoded a polypeptide of 393 amino acids with a deduced molecular mass of 43,962 Da and a calculated pI of 6.74. The primary structure of XylG was 69% similar to that of Thermobifida fusca YX endo-β-1,4-xylanase. It was most active at pH 6.0 and 55 °C. The susceptibilities of xylans to XylG were as follows: oat spelt xylan > birchwood xylan > beechwood xylan. The XylG also showed high activity (474 IU/mg) toward p-nitrophenylcellobioside. Moreover, at pH 6.0 and 50 °C, the Vmax and Km values of the XylG were 127 IU/mg and 2.51 mg/ml, respectively, for oat spelt xylan and 782 IU/mg and 5.26 mM, respectively, for p-nitrophenylcellobioside. A homology model indicated that XylG folded to form a (β/α)8-barrel with two catalytic residues of an acid/base (Glu181) and a nucleophile (Glu289). The formation of a disulfide bond between Cys321 and Cys327 were predicted by homology modeling.
- Keyword
- Endo-β-1,4-xylanasep-NitrophenylcellobiosidePNP-cellobiosideStreptomyces thermocarboxydus HY-15Glycoside hydrolase family 10
- ISSN
- 1381-1177
- Publisher
- Elsevier
- DOI
- http://dx.doi.org/10.1016/j.molcatb.2009.08.015
- Type
- Article
- Appears in Collections:
- Division of Biomedical Research > Microbiome Convergence Research Center > 1. Journal Articles
Division of Bio Technology Innovation > Core Research Facility & Analysis Center > 1. Journal Articles
Jeonbuk Branch Institute > Biological Resource Center > 1. Journal Articles
Synthetic Biology and Bioengineering Research Institute > 1. Journal Articles
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