Processing and subcellular localization of ADAM2 in the Macaca fascicularis testis and sperm

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Title
Processing and subcellular localization of ADAM2 in the Macaca fascicularis testis and sperm
Author(s)
Ekyune Kim; Jae Woong Lee; D C Baek; Sang Rae Lee; Myeong Su Kim; Sang-Hyun Kim; C S Kim; Z Y Ryoo; H S Kang; Kyu Tae Chang
Bibliographic Citation
Animal Reproduction Science, vol. 117, no. 1-2, pp. 155-159
Publication Year
2010
Abstract
Fertilin, a heterodimeric protein complex composed of ADAM1 and ADAM2 located on the sperm surface, is involved in sperm-egg interaction. In our study, we examined the physiological processing and subcellular localization of M. fascicularis ADAM2 during spermatogenesis in the testis and epididymal tract. M. fascicularis ADAM2 was initially synthesized as a 100 kDa precursor in testicular germ cells. After passing into 50 kDa intermediate form in the epididymal tracts, the precursor form was finally processed into a 47 kDa protein in sperm. We found that M. fascicularis ADAM2 is localized on the sperm surface and contributes to the formation of a candidate fertilin complex. In particular, Far-Western blot analysis revealed that M. fascicularis ADAM2 cystein-rich domain may be related to protein-protein interaction. Therefore, the cystein-rich domain of ADAM2 could provide a mechanism to form a fertilin complex.
Keyword
FertilinFertilizationMacaca fascicularis ADAM2Spermatogenesis
ISSN
0378-4320
Publisher
Elsevier
DOI
http://dx.doi.org/10.1016/j.anireprosci.2009.04.002
Type
Article
Appears in Collections:
Ochang Branch Institute > Division of National Bio-Infrastructure > National Primate Research Center > 1. Journal Articles
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